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Series GSE85213 Query DataSets for GSE85213
Status Public on Feb 22, 2018
Title Astrocyte-specific deletion of SOX2 promotes recovery after traumatic brain injury
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Injury to the adult brain induces activation of resident astrocytes, which serves as a compensatory response modulating tissue damage and recovery. However, the mechanism governing astrocyte activation and the role of reactive astrocytes remain largely unknown. Here we show that SOX2, a transcription factor critical for stem cells and brain development, is also required for injury-induced activation of adult cortical astrocytes. Genome-wide ChIP-seq analysis reveals that SOX2 binds to regulatory regions of genes associated with signaling pathways controlling reactive gliosis, such as Socs3, Nr2e1, Notch1, and Akt2. Inducible deletion of Sox2 in adult astrocytes greatly diminishes their response to traumatic injury and, most unexpectedly, restricts injury-induced cortical loss. Together, these results uncover an essential role of SOX2 in terminally differentiated cells and implicate that SOX2-dependent reactive astrocytes may be targeted for regeneration after traumatic brain injury.
Overall design SOX2 (1 input dataset, 3 immunoprecipitation datasets)
Contributor(s) Derek K. S, Chunhai C, Jianjing Y, Chun-Li Z
Citation(s) 29161339
Submission date Aug 04, 2016
Last update date May 15, 2019
Contact name Derek K Smith
Organization name The University of Texas Southwestern Medical Center
Department Molecular Biology
Lab Chun-Li Zhang, Ph.D.
Street address 5323 Harry Hines Blvd, NA5.202
City Dallas
State/province Texas
ZIP/Postal code 75390
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (4)
GSM2261508 Input_SOX2_Mouse_Cortex
GSM2261509 IP1_SOX2_Mouse_Cortex
GSM2261510 IP2_SOX2_Mouse_Cortex
BioProject PRJNA336563
SRA SRP080952

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE85213_RAW.tar 987.1 Mb (http)(custom) TAR (of BEDGRAPH, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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