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Series GSE85303 Query DataSets for GSE85303
Status Public on Aug 10, 2016
Title RNAseq changes in fibroblasts after treatment with selected protein(s) or E16.5 skin extract
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: To identify the cellular processes upregulated in fibroblasts after treatment with selected protein(s) or E16.5 skin extract
Methods: To explore the mechanism of extract-induced HF neogenesis, adult dermal fibroblasts were cultured in the presence of E16.5 extract or selected protein(s) for 3 days before being tested.
Results: we determined how the exposure of adult fibroblasts to E16.5 extract, the selected protein mix or each of the selected proteins and compared the resultant transcriptomes with those of adult DP and embryonic dermal fibroblasts.
Conclusions: Gene expression of adult fibroblasts exposed to E16.5 extract and selected proteins mix, the key DP signature genes became upregulated.
Overall design Fibroblasts mRNA profiles of mouse with different treatments and adult DP and embryonic dermal fibroblasts were generated by next generation sequencing. SRA study accession: SRP081009; BioProject accession: PRJNA337890
Contributor(s) Fan SM, Lin S
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BioProject PRJNA337890
Submission date Aug 08, 2016
Last update date May 15, 2019
Contact name Mai-Yi Fan
Organization name National Taiwan University
Street address No1, Sec. 1, Jen - Ai Rd.
City Taipei
ZIP/Postal code 100
Country Taiwan
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM2264300 sample1: E14.5 FBs
GSM2264301 sample2: DP
GSM2264302 sample3: FBs
SRA SRP081009

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE85303_genes.fpkm_tracking.gz 1.8 Mb (ftp)(http) FPKM_TRACKING
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Raw data are available in SRA
Processed data are available on Series record

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