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Series GSE85344 Query DataSets for GSE85344
Status Public on Aug 11, 2017
Title ChIP-seq data of RedN-venus from asynchronous and swarmer populations of Caulobacter crescentus NA1000
Organism Caulobacter vibrioides NA1000
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Goal: We have discovered and charactherized a new Nucleoid Associated Protein in Caulobacter crescentus, that we named RedN. To determine its global binding on C.crescentus chromosome we have performed a ChIP-seq using a strain with a RedN-Venus fusion expressed at gene loci and as single copy on the chromosome.
 
Overall design We analyzed 2 replicates for the asynchronous population and 1 replicate for the swarmer population after synchrony of RedN-venus expressed in wild type samples (Caulobacter crescentus NA1000). We also performed 1 replicate using Venus protein expressed at xylose locus as control for non-specific binding at regions of high expression, this sample was named Mock. The total DNA before Immunoprecipitation was also extracted and sequenced and used as the control for peak detection for every biological sample, this samples were called Input.
 
Contributor(s) Arias-Cartin R, Dobihal G, Mehta S, Jacobs-Wagner C
Citation(s) 28011580
Submission date Aug 09, 2016
Last update date May 15, 2019
Contact name Christine Jacobs-Wagner
E-mail(s) christine.jacobs-wagner@yale.edu
Phone 203-737-6778
Organization name Yale University
Department Molecular, Cellular and Developmental Biology
Lab Microbial Sciences Institute, Jacobs-Wagner Lab
Street address 840 West Campus Drive, PO BOX 27388
City West Haven
State/province CT
ZIP/Postal code 06516
Country USA
 
Platforms (1)
GPL18276 Illumina HiSeq 2000 (Caulobacter crescentus NA1000)
Samples (8)
GSM2265434 RedN-Venus IP AsyncRep1
GSM2265435 Input AsyncRep1
GSM2265436 RedN-Venus IP AsyncRep2
Relations
BioProject PRJNA338232
SRA SRP081124

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Supplementary file Size Download File type/resource
GSE85344_RAW.tar 167.9 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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