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Series GSE85366 Query DataSets for GSE85366
Status Public on Aug 10, 2016
Title BCR repertoire analyis of class-switched and non-switched thymic B cells
Organism Mus musculus
Experiment type Other
Summary We report that a large percentage of thymic B cells undergo class switching intrathymically. Thymic B cell class switching requires cognate T-B interaction. To determine whether B cell specificity was also important for thymic B cell class-switching, we sorted class-switched thymic B cells (CD19+B220+IgM-IgD-), unswitched B cells (CD19+B220+IgM+IgD+) and bulk splenic B cells in 3H9 heavy chain-fixed mice and performed high throughput sequencing analysis of the light chain of these populations. Results of this analysis indicated that class-switched thymic B cells have a distinct repertoire compared with unswitched thymic B cells and splenic B cells. Further reactivity tests indicated that a large part of BCRs enriched in class-switched thymic B cells are autoreactive. These data suggest that autoreactive B cells are selected into class-switched population and expanded in the thymus.
 
Overall design Light chain repertoire profiles of class-switched thymic B cells, unswithced thymic B cells and splenic B cells from 3H9 mice were generated by deep sequencing.
 
Contributor(s) Perera J, Huang H
Citation(s) 27705788
Submission date Aug 09, 2016
Last update date May 15, 2019
Contact name Zhong Zheng
E-mail(s) zzheng16@medicine.bsd.uchicago.edu
Phone 7738344481
Organization name University of Chicago College of Medicine
Department Section of Rheumatology
Lab Haochu Huang Lab
Street address 924 E57th ST, Room 402
City Chicago
State/province IL
ZIP/Postal code 60637
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (19)
GSM2265875 SW1
GSM2265876 SW2
GSM2265877 SW3
Relations
BioProject PRJNA338285
SRA SRP081145

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Supplementary file Size Download File type/resource
GSE85366_3H9Usage.xlsx 3.7 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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