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Series GSE85366 Query DataSets for GSE85366
Status Public on Aug 10, 2016
Title BCR repertoire analyis of class-switched and non-switched thymic B cells
Organism Mus musculus
Experiment type Other
Summary We report that a large percentage of thymic B cells undergo class switching intrathymically. Thymic B cell class switching requires cognate T-B interaction. To determine whether B cell specificity was also important for thymic B cell class-switching, we sorted class-switched thymic B cells (CD19+B220+IgM-IgD-), unswitched B cells (CD19+B220+IgM+IgD+) and bulk splenic B cells in 3H9 heavy chain-fixed mice and performed high throughput sequencing analysis of the light chain of these populations. Results of this analysis indicated that class-switched thymic B cells have a distinct repertoire compared with unswitched thymic B cells and splenic B cells. Further reactivity tests indicated that a large part of BCRs enriched in class-switched thymic B cells are autoreactive. These data suggest that autoreactive B cells are selected into class-switched population and expanded in the thymus.
Overall design Light chain repertoire profiles of class-switched thymic B cells, unswithced thymic B cells and splenic B cells from 3H9 mice were generated by deep sequencing.
Contributor(s) Perera J, Huang H
Citation(s) 27705788
Submission date Aug 09, 2016
Last update date May 15, 2019
Contact name Zhong Zheng
Phone 7738344481
Organization name University of Chicago College of Medicine
Department Section of Rheumatology
Lab Haochu Huang Lab
Street address 924 E57th ST, Room 402
City Chicago
State/province IL
ZIP/Postal code 60637
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (19)
GSM2265875 SW1
GSM2265876 SW2
GSM2265877 SW3
BioProject PRJNA338285
SRA SRP081145

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Supplementary file Size Download File type/resource
GSE85366_3H9Usage.xlsx 3.7 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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