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| Status |
Public on Aug 10, 2019 |
| Title |
Perinatal Exposure to 2,2’,4’4’ –Tetrabromodiphenyl Ether Impairs Male Reproductive Health in Adult Rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Toxicity of PBDE for male reproductive system was shown in several human and animal studies, however long lasting effects of perinatal exposures on male reproduction are yet poorly understood. In this study pregnant Wistar rats were exposed to 0.2 mg/kg 2,2’,4,4’-tetrabromodiphenyl ether (BDE-47) from gestation day 8 till postnatal day 21 and testis transcriptome was analyzed on postnatal day 120 in offspring. Exposed animals had significant change in testes transcriptome including suppression of genes essential for spermatogenesis and activation of immune response genes. In particular exposed animals had on average 4 fold decreased expression of protamine and transition protein genes in testes suggesting that histone-protamine exchange may be dysregulated in the course of spermatogenesis resulting in exposure legacy transfer to the next generation via aberrant sperm epigenome.
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| Overall design |
Rats were exposed perinataly to vehicle or BDE-47. Total RNA was extracted from testis of 5 control and 5 exposed animals on postnatal day 120 and sequenced with multiplexing on NextSeq500.
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| Contributor(s) |
Suvorov A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 10, 2016 |
| Last update date |
Aug 10, 2019 |
| Contact name |
Alexander Suvorov |
| E-mail(s) |
asuvorov@schoolph.umass.edu
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| Organization name |
University of Massachusetts
|
| Department |
Environmental Health Sciences
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| Street address |
149A Goessman, 686 N.Pleasant Str
|
| City |
Amherst |
| State/province |
MA |
| ZIP/Postal code |
01003-9303 |
| Country |
USA |
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| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA338429 |
| SRA |
SRP081216 |
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