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Series GSE85711 Query DataSets for GSE85711
Status Public on May 01, 2017
Title Inhalation and oropharyngeal aspiration exposure to rod-like carbon nanotubes induces highly similar airway inflammation and biological pathways in mouse lungs
Organism Mus musculus
Experiment type Expression profiling by array
Summary Inhalation and low-dose aspiration elicit highly similar pulmonary inflammation which was confirmed by activation of essentially similar biological pathways and even the same genes. Present results provide evidence that oropharyngeal aspiration is valid approach for hazard assessment of nanomaterials and proper alternative method for inhalation exposure.
Overall design To compare two alternative airway exposure methods, C57BL/6 mice were exposed to rCNT by inhalation method (aerosol mass concentrations of 6.2-8.2 mg/m3; 4h/day for 4 days), or by oropharyngeal aspiration. Low-dose (10 ug/day in 50 ul of PBS ) or high-dose (40 ug/day in 50ul of PBS) aspiration exposure of rCNT was used for four days. Bronchoalveolar lavage and lung tissue samples were collected, histological sections analyzed and mRNA expression profiles were determined by Agilent microarray. Untreated mice were used as Controls for inhalation, and vehichle treated (PBS) as Controls for aspiration.
Contributor(s) Kinaret P, Ilves M, Fortino V, Rydman E, Piia K, Wolff H, Savolainen K, Greco D, Alenius H
Citation(s) 28045493
Submission date Aug 16, 2016
Last update date Jul 19, 2017
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platforms (1)
GPL13912 Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version)
Samples (15)
GSM2282060 rCNT aspiration 4days + 24h M1_4d24h
GSM2282061 rCNT aspiration 4days + 24h M3_4d24h
GSM2282062 Vehicle (PBS) aspiration 4days + 24h C2_4d24h
BioProject PRJNA339197

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Supplementary file Size Download File type/resource
GSE85711_RAW.tar 99.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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