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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 31, 2017 |
| Title |
RNA-seq analysis of Jak1 KO and wt LT-HSCs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Previous studies have illustrated the importance of Jak1-mediated cytokine signaling in immunological and neoplastic diseases such that JAK1 inhibition is currently being investigated in clinical trials. However, the role of Jak1 in hematopoietic stem cell (HSC) function has not been delineated. Here we show that conditional Jak1 loss in hematopoietic cells reduces HSCs and markedly alters lymphoid/myeloid differentiation in vivo. Jak1-deficient stem cells exhibit decreased competitiveness in vivo, and are unable to rescue hematopoiesis in the setting of myelosuppression. Jak1-deficient HSCs show increased quiescence, an inability to enter the cell cycle in response to hematopoietic stress, and do not respond to type I interferons demonstrating Jak1 as a central node for interferon signaling in HSCs. Moreover, the HSC defect induced by Jak1 loss is not fully rescued by expression of a constitutively active Jak2 allele. Our data highlights an essential role for Jak1 in HSC homeostasis and stress response.
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| Overall design |
6 samples per group; group 1: wt, group 2: KO
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| Contributor(s) |
Kleppe M, Spitzer MH, Li S, Dong L, Papalexi E, Hill C, De Groote S, Keller M, Koppikar P, Rapaport FT, Gandara J, Mason CE, Nolan GP, Levine RL |
| Citation(s) |
28965767 |
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| Submission date |
Aug 17, 2016 |
| Last update date |
Jul 31, 2019 |
| Contact name |
Christopher E Mason |
| E-mail(s) |
chm2042@med.cornell.edu
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| Organization name |
Weill Cornell Medical College
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| Department |
Physiology and Biophysics
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| Lab |
Christopher Mason Lab
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| Street address |
1305 York Ave
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| City |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA339238 |
| SRA |
SRP082367 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE85745_mm10_gene_count.txt.gz |
453.0 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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