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Series GSE85875 Query DataSets for GSE85875
Status Public on Feb 03, 2017
Title Single cell transcriptome sequencing of mammary stem cells in the pubertal mammary gland
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The mammary gland is a highly dynamic organ that mainly develops during puberty. Based on morphology and proliferation analysis, mammary stem cells (MaSCs) are thought to be close to or reside in the terminal end buds (TEBs) during pubertal development. However, exclusive stem cell markers are lacking, and therefore the true identity of MaSCs, including their location, multiplicity, dynamics and fate during branching morphogenesis, has yet to be defined. To gain more insights into the molecular identity and heterogeneity of the MaSC pool, we performed single cell transcriptome sequencing of mammary epithelial cells micro-dissected from ducts and TEBs during puberty. These data show that the behaviour of MaSCs cannot be directly linked to a single expression profile. Instead, morphogenesis of the mammary epithelium relies upon a heterogeneous population of MaSCs that functions long-term as a single equipotent pool of stem cells.
Overall design Ducts and terminal end buds were micro-dissected from the 4th and the 5th murine mammary gland at 5 weeks-of-age, dissociated into single cells, and FACS sorted. Single-cell transcriptomics was performed on live cells using an automated version of CEL-seq2 on live, FACS sorted cells. The StemID algorithm was used to identify clusters of cells corresponding to basal and luminal cells types derived from ducts and terminal end buds.
Contributor(s) Scheele CJ, Muraro MJ, van Rheenen J, van Oudenaarden A
Citation(s) 28135720
Submission date Aug 20, 2016
Last update date May 15, 2019
Contact name Mauro Muraro
Organization name Single Cell Discoveries
Street address Uppsalalaan, 8
City Utrecht
State/province Utrecht
ZIP/Postal code 3584 CT
Country Netherlands
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM2286438 mammary epithelial cells, plate 1, library 1
GSM2286439 mammary epithelial cells, plate 1, library 2
GSM2286440 mammary epithelial cells, plate 1, library 3
BioProject PRJNA339617
SRA SRP082453

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Supplementary file Size Download File type/resource
GSE85875_J2_mammary_gland_singlecells.csv.gz 1.2 Mb (ftp)(http) CSV
GSE85875_readme_demultiplexing_Cel-seq_data.pdf 222.5 Kb (ftp)(http) PDF
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Raw data are available in SRA
Processed data are available on Series record

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