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| Status |
Public on Mar 15, 2008 |
| Title |
High-resolution genetic analysis of cancer cell lines with near normal karyotypes |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by SNP array
SNP genotyping by SNP array
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| Summary |
Cancer development is associated with multiple genetic alterations and genomic instability either at the chromosomal or base pair level is generally thought to underlie these changes. However, it is still unknown whether genetic instability is absolutely required for tumorigenesis. Here we investigated the genomic instability status of four cytogenetically stable diploid cell lines CAL51, SK-UT-1B, A204 and CH1. We applied high resolution 500K single nucleotide polymorphism (SNP) array analysis and found that all the four cell lines have some sub-microscopic genomic copy number changes. Interestingly, there were many more sub-microscopic chromosome alterations in A204 and CH1 than in CAL51 and SK-UT-1B. Twenty-four-color fluorescence in situ hybridization (FISH) was used to analyse a large number of metaphases from CAL51, SK-UT-1B and CH1 cells. The rate of de novo chromosome rearrangements was significantly higher in CH1 than CAL51 and SK-UT-1B. Although this increased rate did not lead to many clonal cytogenetically apparent chromosome alterations in CH1 cells, it is consistent with a first step towards chromosomal instability. It is more striking that both cell lines CAL51 and SK-UT-1B which have a similar de novo chromosomal change rate to that of normal lymphocytes were microsatellite instability positive by BAT-26 microsatellite analysis. This study further strengths the current concept that genomic instability is associated with tumor development.
Keywords: DNA copy number changes
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| Overall design |
DNA from A204, CH1, CAL51 and SK-UT-1B were analyzed by Affymetrix Genechip Mapping 500K Set array. Data were compared with normal samples from HAPMAP database.
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| Contributor(s) |
Mao X, Young BD, Chaplin T, Shipley J, Lu Y |
| Citation(s) |
18407410 |
| |
| Submission date |
Aug 01, 2007 |
| Last update date |
May 17, 2017 |
| Contact name |
Xueying Mao |
| E-mail(s) |
x.mao@qmul.ac.uk
|
| Organization name |
Queen Mary
|
| Street address |
Charterhouse Square
|
| City |
London |
| ZIP/Postal code |
EC1M 6BQ |
| Country |
United Kingdom |
| |
|
| Platforms (2) |
| GPL3718 |
[Mapping250K_Nsp] Affymetrix Mapping 250K Nsp SNP Array |
| GPL3720 |
[Mapping250K_Sty] Affymetrix Mapping 250K Sty2 SNP Array |
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| Samples (8)
|
| GSM214534 |
Human rhabdomyosarcoma cell line_A204_NSP |
| GSM214535 |
Human rhabdomyosarcoma cell line_A204_STY |
| GSM214536 |
Human ovarian carcinoma cell line_CH1_NSP |
| GSM214537 |
Human ovarian carcinoma cell line_CH1_STY |
| GSM214538 |
Human breast carcinoma cell line_CAL51_NSP |
| GSM214539 |
Human breast carcinoma cell line_CAL51_STY |
| GSM214540 |
Human leiomyosarcoma cell line_SKUT1B_NSP |
| GSM214541 |
Human leiomyosarcoma cell line_SKUT1B_STY |
|
| Relations |
| BioProject |
PRJNA101839 |
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