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Series GSE86583 Query DataSets for GSE86583
Status Public on Dec 12, 2017
Title Sexual-lineage-specific DNA methylation regulates meiosis in Arabidopsis
Organism Arabidopsis thaliana
Experiment type Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary We report a distinctive gene-targeted RNA-directed DNA methylation (RdDM) activity in the Arabidopsis thaliana male sexual lineage that regulates gene expression in meiocytes. Loss of sexual-lineage-specific RdDM causes mis-splicing of the MPS1 gene (also known as PRD2), thereby disrupting meiosis. Our results establish a regulatory paradigm in which de novo methylation creates a cell-lineage-specific epigenetic signature that controls gene expression and contributes to cellular function in flowering plants.
 
Overall design Whole genome bisulfite sequencing and RNA-seq of wild type and RdDM mutant arabidopsis male sex cells and somatic tissues
 
Contributor(s) Walker J, Gao H, Zhang J, Aldridge B, Vickers M, Higgins J, Feng X
Citation(s) 29255257
Submission date Sep 08, 2016
Last update date May 15, 2019
Contact name Xiaoqi Feng
E-mail(s) xiaoqi.feng@jic.ac.uk
Organization name John Innes Centre
Department Cell and Developmental Biology
Lab Xiaoqi Feng
Street address Norwich Research Park
City Norwich
State/province Norfolk
ZIP/Postal code NR4 7UH
Country United Kingdom
 
Platforms (3)
GPL13222 Illumina HiSeq 2000 (Arabidopsis thaliana)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
GPL21785 Illumina HiSeq 4000 (Arabidopsis thaliana)
Samples (20)
GSM2306307 drm1drm2_meiocyte_BSseq_rep1
GSM2306308 drm1drm2_meiocyte_BSseq_rep2
GSM2306309 drm1drm2_sperm_BSseq
Relations
BioProject PRJNA342309
SRA SRP087631

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE86583_GEO_submission_tableS2.xlsx 44.9 Kb (ftp)(http) XLSX
GSE86583_RAW.tar 2.6 Gb (http)(custom) TAR (of GFF)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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