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| Status |
Public on Jun 12, 2017 |
| Title |
Changes in gene expression upon treatment of SH-SY5Y cells to cisplatin |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In this study we investigated the changes in mRNA expression upon treatment of SH-SY5Y cells to 10µM cisplatin for 72h.
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| |
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| Overall design |
Three samples of parental SH-SY5Y cells and four samples of 72h treated SH-SY5Y neuro-blastoma cell line with 10µM CDDP were subjected to Affymetrix chip analyses. RNA quality was determined using the Agilent Bioanalyzer and the Eukaryote Total RNA Nano Chip (Ag-ilent Technologies, Hayward, CA, USA). The RNA integrity number (RIN) was measured as 9.9-10. The Affymetrix HuGene 2.0 ST and WT plus reagent kit (Affymetrix, Santa Clara, California, United States) was used following the provider’s recommendation with a start ma-terial of 100ng of total RNA.
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| |
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| Contributor(s) |
Florea A, Sass S |
| Citation(s) |
28206967 |
| |
| Submission date |
Sep 12, 2016 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Ana-Maria Florea |
| E-mail(s) |
anamflorea@gmail.com
|
| Phone |
+491797776429
|
| Organization name |
University Düsseldorf
|
| Department |
Neuropathology
|
| Lab |
Ana-Maria Florea
|
| Street address |
Moorenstr. 5
|
| City |
Düsseldorf |
| State/province |
NRW |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA342673 |
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