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Series GSE86842 Query DataSets for GSE86842
Status Public on Jun 12, 2017
Title Changes in gene expression upon treatment of SH-SY5Y cells to cisplatin
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In this study we investigated the changes in mRNA expression upon treatment of SH-SY5Y cells to 10µM cisplatin for 72h.
Overall design Three samples of parental SH-SY5Y cells and four samples of 72h treated SH-SY5Y neuro-blastoma cell line with 10µM CDDP were subjected to Affymetrix chip analyses. RNA quality was determined using the Agilent Bioanalyzer and the Eukaryote Total RNA Nano Chip (Ag-ilent Technologies, Hayward, CA, USA). The RNA integrity number (RIN) was measured as 9.9-10. The Affymetrix HuGene 2.0 ST and WT plus reagent kit (Affymetrix, Santa Clara, California, United States) was used following the provider’s recommendation with a start ma-terial of 100ng of total RNA.
Contributor(s) Florea A, Sass S
Citation(s) 28206967
Submission date Sep 12, 2016
Last update date Mar 15, 2019
Contact name Ana-Maria Florea
Phone +491797776429
Organization name University Düsseldorf
Department Neuropathology
Lab Ana-Maria Florea
Street address Moorenstr. 5
City Düsseldorf
State/province NRW
ZIP/Postal code 40225
Country Germany
Platforms (1)
GPL16686 [HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version]
Samples (7)
GSM2309282 control 1
GSM2309283 control 2
GSM2309284 control 3
BioProject PRJNA342673

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Supplementary file Size Download File type/resource
GSE86842_RAW.tar 58.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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