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| Status |
Public on Jan 23, 2017 |
| Title |
miR-182 regulates Slit2-mediated axon guidance by modulating the local translation of a specific mRNA |
| Organism |
Xenopus laevis |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
During brain wiring, mRNAs are trafficked into axons and growth cones where they are differentially translated in response to extrinsic signals. Differential control of local protein synthesis mediates neuronal compartment-specific behaviors that aid axon guidance. Yet little is understood about how specific mRNAs are selected for translation. Here we have investigated the local role of microRNAs (miRNAs) in mRNA-specific translation during axon pathfinding of Xenopus laevis retinal ganglion cell (RGC) axons. Profiling experiments revealed a rich repertoire of axonal miRNAs in developing RGC axons and identified miR-182 as one of the most abundant. Loss of miR182 impairs Slit2-induced growth cone repulsion and causes RGC axon targeting defects in vivo. To aid miRNA target prediction, we also profiled mRNA expression in RGC axons. Our results show that miR-182 targets cofilin1 mRNA in RGC growth cones and modulates its local translation in response to Slit2.
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| Overall design |
Two biological replicates, one condition
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| Contributor(s) |
Bellon A, Iyer A, Bridi S, Lee F, Ovando-Vázquez C, Corradi E, Longhi S, Rocuzzo M, Strohbuecker S, Naik S, Sarkies P, Miska E, Abreu-Goodger C, Holt CE, Baudet M |
| Citation(s) |
28147273 |
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| Submission date |
Sep 13, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Cei Abreu-Goodger |
| E-mail(s) |
cei.abreu@cinvestav.mx
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| Organization name |
Langebio - Cinvestav
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| Street address |
Km. 9.6 Libramiento Nte Carr Leon-Irapuato
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| City |
Irapuato |
| State/province |
Guanajuato |
| ZIP/Postal code |
36824 |
| Country |
Mexico |
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| Platforms (1) |
| GPL22426 |
Illumina MiSeq (Xenopus laevis) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA342827 |
| SRA |
SRP089815 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE86883_RAW.tar |
17.8 Mb |
(http)(custom) |
TAR (of FA) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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