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Series GSE87009 Query DataSets for GSE87009
Status Public on Jun 12, 2017
Title Quorum Sensing/Quorum Quenching in Acinetobacter baumannii clinical strains
Organism Acinetobacter baumannii
Experiment type Expression profiling by array
Summary We worked with microarrys analysis in presence of 3-oxo-C12-HSL molecule to analyze the profile of the genes implicated in the Quorum Quenching network in A.baumannii clinical strains. Interestingly, only 13 genes were overexpressed under 3-oxo-C12-HSL molecule being the most level a gene which encodes an Alpha/beta hydrolase enzyme (5.01). The 46.15% of the genes overexpressed were implicated in the synthesis of the acyl-homoserine lactones (AHLs).
 
Overall design The arrays were designed (Bioarray Genetic Diagnosis, Alicante) using eArray (Agilent) from the complete genome of A. baumannii strain (Ab ST-2 clon_2010). Experiments were performed from three biological replicates of RNA treated with DNAse obtained from a culture in exponential average phase (optical density at 600 nm of 0.6) in the presence of 3-oxo-C12- HSL at a final concentration of 10 uM. As a negative control (RNA untreated) RNA was used with dimethyl sulfoxide (DMSO, a solvent of said acyl homoserine lactone). The RNA samples were quantified in a NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies). Quality and integrity analysis of the samples was carried out in an Agilent 2100 Bioanalyzer with the RNA 6000 Nano reagents and the RNA Nano Chips (Agilent Technologies).
 
Contributor(s) Lopez M, Gato E, Blasco L, Fernandez-Garcia L, Bou G, Tomas M
Citation(s) 28328989
Submission date Sep 16, 2016
Last update date Dec 18, 2017
Contact name Maria Tomas-Carmona
E-mail(s) ma.del.mar.tomas.carmona@sergas.es
Organization name Instituto de Investigación Biomédica de A Coruña (INIBIC) / Complexo Hospitalario Universitario de A Coruña (CHUAC)
Department Microbiology Department
Street address As Xubias, 84
City A Coruña
ZIP/Postal code 15006
Country Spain
 
Platforms (1)
GPL20821 Agilent-068651 MCCarmona v1
Samples (4)
GSM2318806 C12_0.5 vs DMSO_0.5 1
GSM2318807 C12_0.5 vs DMSO_0.5 2
GSM2318808 DMSO_0.5 vs C12_0.5 1
Relations
BioProject PRJNA343222

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE87009_RAW.tar 6.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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