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Status |
Public on Mar 07, 2017 |
Title |
Global transcriptional response in Japanese medaka larvae following embryonic exposure to TCDD |
Organism |
Oryzias latipes |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
A global RNA-Seq analysis was conducted using RNA isolated from the axial region of DMSO- and 0.3 nM TCDD-exposed medaka to identify targets within the osteochondral pathway potentially impacted by TCDD exposure. In total, 597 genes were significantly up- or down-regulated (q<0.05) and were associated with select pathological states including inflammatory disease, connective tissue disorders, and skeletal and muscular disorders.
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Overall design |
RNA was isolated from axial tissue (containing vertebrae and surrounding muscle, fat, and dermis) from Japanese medaka larvae. Whole transcriptome libraries from DMSO and TCDD-medaka were generated for downstream RNA-Seq using the Ion Torrent PGM platform.
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Contributor(s) |
Watson A, Kullman S |
Citation(s) |
28077779 |
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Submission date |
Sep 21, 2016 |
Last update date |
May 15, 2019 |
Contact name |
AtLee Watson |
E-mail(s) |
atwatson@ncsu.edu
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Organization name |
North Carolina State University
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Street address |
Campus Box 7633
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City |
Raleigh |
State/province |
North Carolina |
ZIP/Postal code |
27695 |
Country |
USA |
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Platforms (1) |
GPL22469 |
Ion Torrent PGM (Oryzias latipes) |
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Samples (6)
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Relations |
BioProject |
PRJNA343774 |
SRA |
SRP090229 |