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Series GSE87170 Query DataSets for GSE87170
Status Public on Dec 13, 2016
Title DNA methylation inheritance across generations
Organism Arabidopsis thaliana
Experiment type Methylation profiling by high throughput sequencing
Summary Cytosine DNA methylation regulates the expression of eukaryotic genes and transposable elements. Methylation is copied by DNA methyltransferases after DNA replication, which results in faithful transmission of methylation patterns during cell division and, at least in flowering plants, across generations. Trans-generational inheritance is mediated by a small group of cells that includes gametes and their progenitors. However, methylation is usually analyzed in somatic tissues that do not contribute to the next generation, and the mechanisms of trans-generational inheritance are inferred from such studies. To gain a better understanding of how DNA methylation is inherited, we analyzed purified Arabidopsis thaliana sperm and vegetative cells – the cell types that comprise pollen – with mutations in the DRM, CMT2 and CMT3 methyltransferases.
Overall design We isolated sperm and vegetative nuclei from pollen of Col-0, and cmt2, cmt3, drm1 drm2 (d1d2), and h1.1 h1.2 (h1) mutant Arabidopsis thaliana plants, respectively. DNA is extracted from the isolated nuclei and used for bisulfite-sequencing.
Contributor(s) Hsieh P, He S, Buttress T, Gao H, Couchman M, Fischer RL, Zilberman D, Feng X
Citation(s) 27956643
Submission date Sep 21, 2016
Last update date May 15, 2019
Contact name Xiaoqi Feng
Organization name John Innes Centre
Department Cell and Developmental Biology
Lab Xiaoqi Feng
Street address Norwich Research Park
City Norwich
State/province Norfolk
ZIP/Postal code NR4 7UH
Country United Kingdom
Platforms (2)
GPL13222 Illumina HiSeq 2000 (Arabidopsis thaliana)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (10)
GSM2323840 c2_Sperm
GSM2323841 c2_Vegetative
GSM2323842 c3_Sperm
BioProject PRJNA343778
SRA SRP090231

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Supplementary file Size Download File type/resource
GSE87170_RAW.tar 3.2 Gb (http)(custom) TAR (of GFF)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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