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Series GSE87347 Query DataSets for GSE87347
Status Public on Apr 14, 2017
Title Expression profiling of microRNAs in Bovine ovarian follicular development; comparative analyses between LHF and CL groups
Platform organisms Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Merkel cell polyomavirus; Mus musculus cytomegalovirus 2; Betapolyomavirus hominis; Betapolyomavirus macacae
Sample organism Bos taurus
Experiment type Non-coding RNA profiling by array
Summary The study aimed to identify miRNAs expression profiles associated with growth and regression of dominant-size follicles in bovine. Follicles were collected from abattoir ovaries and their status (healthy/atretic) was assessed by measuring steroid levels and aromatase expression. Corpora lutea (CL) were collected from ovarian pairs correponding to days 1 to 4 of the oestrus cycle. Total RNA was isolated from whole follicles at different developmental stages and from CL. An heterologous microarray (Exiqon, Denmark) approach followed by RT-qPCR validation (Qiagen, UK) was used to identify and compare miRNA profiles between large healthy follicles (diameter, 13–16 mm, n=6) and each of small (4–8 mm, n=6 pools of follicles), large atretic folllicles (13-16 mm, n=6) and CL (n=6). RNA from the above groups was hybridized to the miRCURY LNA™ microRNA Hi-Power Labeling Kit,Hy3™/Hy5™ (Exiqon) and hybridized on the miRCURY LNA™ microRNA Array (6th gen). A total of 17 and 57 microRNAs were differentially expressed (> 2 fold, adj. P-value < 0.05) between Large Healthy and each of Small and Large Atretic follicles, respectively, a fraction of which corresponded to registered bovine miRNA sequences. A subset of 5 bovine miRNAs (miR-144, miR-202,vmiR-451, miR-652, miR-873) were confirmed by qPCR to be upregulated in Large Healthy follicles, were enriched in mural granulosa cells and their predicted targets mapped to genes involved in follicular cell proliferation and differentiation, suggesting an involvemet of this subset of microRNAs in ovarian follicle development. In addition, the miR-183-96-183 cluster was identified as upregulated in CL and was shown to be involved in luteal survival and progesterone production.
 
Overall design Six biological replicates per developmental stage (total of 24 samples) were used in a double dye microRNA microarray experiment. Samples were distributed among slides so that each experimental group was represented at least once in each slide. For each gene, mean normalized intensities (n= 6 biological replicates/group) were compared between follicle stages (SF vs LHF, LHF vs LAF and LHF vs CL).
 
Contributor(s) Donadeu FX, Sontakke SD
Citation(s) 28368475
Submission date Sep 26, 2016
Last update date Jul 17, 2017
Contact name Xavier Donadeu
E-mail(s) xavier.donadeu@roslin.ed.ac.uk
Organization name Roslin Institute, University of Edinburgh
Street address Easter Bush
City Midlothian
ZIP/Postal code EH25 9RG
Country United Kingdom
 
Platforms (1)
GPL11434 miRCURY LNA microRNA Array, 6th generation - hsa, mmu & rno
Samples (24)
GSM1322248 bSF_1
GSM1322249 bSF_2
GSM1322250 bSF_3
Relations
BioProject PRJNA344470

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE87347_RAW.tar 52.1 Mb (http)(custom) TAR (of TXT)
GSE87347_bCL_processed_data.txt.gz 12.6 Kb (ftp)(http) TXT
Processed data included within Sample table

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