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| Status |
Public on Jan 25, 2017 |
| Title |
ARGONAUTE10 promotes the degradation of miR165/6 through the SDN1 and SDN2 exonucleases in Arabidopsis [AGO10SDN] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
The degradation of small RNAs in plants and animals is associated with small RNA 3’ truncation and 3’ uridylation and thus relies on exonucleases and nucleotidyl transferases. Argonaute proteins associate with small RNAs in vivo and are essential for not only the activities but also the stability of small RNAs. Arabidopsis ARGONAUTE10 (AGO10) maintains stem cell homeostasis in meristems by sequestration of miR165/6, a conserved miRNA acting through AGO1. Here, we report that AGO10 reduces miR165/6 accumulation by enhancing its degradation. This is contrary to the stabilizing effect of all known argonaute proteins on their associated small RNAs. We show that SMALL RNA DEGRADING NUCLEASES (SDNs) are responsible for miRNA 3’ truncation in general and AGO10 promotes the degradation of miR165/6 by SDNs. Our work identifies the exonucleases responsible for miRNA 3’ truncation in vivo and uncovers a mechanism of specificity determination in miRNA turnover. This work, together with previous studies on AGO10, demonstrates that spatially regulated miRNA degradation underlies stem cell maintenance in plants.
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| Overall design |
Immunoprecipitation of AGO1- and AGO10-RISCs followed by treatment with SDN/SDN-D283A exonucleases (two biological replicates and two treatments for a total of 8 samples).
Immunoprecipitation of AGO1- and AGO10-RISCs followed by Mock treatment that included buffer only (i.e., no SDN) (two biological replicates, a total of 4 samples).
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| Contributor(s) |
Ji L, Yu Y, Le BH, Chen X |
| Citation(s) |
28231321 |
| |
| Submission date |
Sep 26, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Meera Nair |
| E-mail(s) |
meera.nair@ucr.edu
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| Phone |
951-827-7734
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| Organization name |
University of California, Riverside
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| Department |
Biomedical Sciences
|
| Lab |
3135 Multidisciplinary Building
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| Street address |
3401 Watkins Drive
|
| City |
Riverside |
| State/province |
CA |
| ZIP/Postal code |
92521 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE87355 |
ARGONAUTE10 promotes the degradation of miR165/6 through the SDN1 and SDN2 exonucleases in Arabidopsis |
|
| Relations |
| BioProject |
PRJNA344474 |
| SRA |
SRP090467 |
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