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| Status |
Public on Jan 19, 2018 |
| Title |
The transcription factor Gli3 promotes differentiation from double positive to CD4 single positive thymocyte by repression of Shh |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We used Affymetrix microarrays to understand the genome wide differences in Wildtype and Gli3 mutant (Gli3+/- and Gli3-/-) (n=2) embryonic day 18.5 DP CD69-, DP CD69+ and SP4 thymocytes.
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| Overall design |
Wildtype and Gli3 mutant (Gli3+/- and Gli3-/-) (n=2) embryonic day 18.5 fetal thymus were sorted for DP69- (CD4+CD8+CD69-), DP69+ (CD4+CD8+CD69+) and SP4 (CD4+CD8-) cells. RNA extracted from these cells was sequenced to help understand the transcriptional changes governing DP to SP thymocyte differentiation and selection in the Gli3 mutant.
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| Contributor(s) |
Solanki A, Crompton T |
| Citation(s) |
29361554 |
| |
| Submission date |
Sep 29, 2016 |
| Last update date |
Apr 20, 2018 |
| Contact name |
Tessa Crompton |
| Organization name |
UCL
|
| Department |
Immunobiology
|
| Street address |
30 Guilford Street
|
| City |
London |
| ZIP/Postal code |
WC1N 1EH |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA344924 |
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