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Series GSE87588 Query DataSets for GSE87588
Status Public on Jan 01, 2017
Title Restoring miR-150-5p to an estrogen receptor positive breast cancer cell line [ZR-75-1]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The objective of these experiments is to identify novel direct and indirect targets of miR-150-5p in breast cancer cell lines. The goal is that these will give direction as to what targets or pathways may be contributing to the reduced growth observed in these cell lines upon restoration of miR-150-5p. A therapy directed towards one or more critical subtype-specific targets could be developed as a therapeutic for breast cancer patients.
Using has-miR-150-5p mirVana miRNA mimic (Ambion, 4464066), miR-150-5p was restored to an estrogen receptor positive breast cancer cell line, ZR-75-1.
 
Overall design Cells were transfected in triplicate for 48 hours with either mock, negative control, or mimic, and RNA isolated from each sample was hybridized onto Affymetrix GeneChip® Human Gene Array 2.0 ST ( probesets).
 
Contributor(s) Heinz R, Spoelstra NS, Gu H, Richer JK
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Oct 04, 2016
Last update date Mar 15, 2019
Contact name Jennifer K Richer
E-mail(s) jennifer.richer@ucdenver.edu
Organization name University of Colorado Anschutz Medical Campus
Department Pathology
Lab Richer Lab
Street address Mail Stop 8104, RC1N 5th Floor P18-5127, 12800 E. 19th Ave.
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
 
Platforms (1)
GPL16686 [HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version]
Samples (9)
GSM2334848 ZR-75-1_Mock 2-1
GSM2334849 ZR-75-1_Mock 2-2
GSM2334850 ZR-75-1_Mock 2-3
This SubSeries is part of SuperSeries:
GSE87590 Restoring miR-150-5p to breast cancer cell lines
Relations
BioProject PRJNA345315

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE87588_RAW.tar 71.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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