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Series GSE89064 Query DataSets for GSE89064
Status Public on Jun 12, 2017
Title Host genetic background strongly affects pulmonary miRNA expression before and during evolution of influenza A virus infection
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Background: The role of host encoded microRNAs in genetically determined host susceptibility to influenza A virus (IAV) infection has not been explored. We therefore compared changes in pulmonary miRNA expression during IAV infection in two inbred mouse strains with differential susceptibility to IAV infection.

Results: miRNA expression profiles were determined in lungs of the more susceptible mouse strain DBA/2J and the less susceptible strain C57BL/6J within 120 hours post infection (hpi) with IAV (H1N1) PR8. Even the miRNomes of uninfected lungs differed substantially between the two strains. After a period of relative quiescence, major miRNome reprogramming was detected in both strains by 48 hpi and increased through 120 hpi. Distinct groups of miRNAs regulated by IAV infection could be defined: (1) miRNAs (n= 39) whose expression correlated with HA mRNA expression and represented the general response to IAV infection independent of host genetic background; (2) miRNAs (n=20) whose expression correlated with HA mRNA expression but differed between the two strains; and (3) remarkably, miRNAs (n=8) whose abundance even in uninfected lungs differentiated nearly perfectly (area under the ROC curve >0.99) between the two strains throughout the time course. Higher abundance of miRNAs with anti-apoptotic functions and lower abundance of miRNAs regulating the PI3K-Akt pathway were associated with the more susceptible DBA/2J strain.

Conclusions: These results suggest that differences in host miRNA abundance before and during IAV infection are in part determined genetically and contribute to susceptibility to IAV infection in this experimental mouse model, and likely in humans.
Overall design Influenza A virus infection timecourse in two mouse strains (C57BL/6J, DBA/2J) with different susceptibility to the virus.
Contributor(s) Preusse M, Schughart K, Pessler F
Citation(s) 28377766
Submission date Oct 22, 2016
Last update date May 15, 2019
Contact name Matthias Preusse
Organization name Helmholtz Centre for Infection Research
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (125)
GSM2358180 Lung from untreated C57BL/6J mouse, rep1
GSM2358181 Lung from untreated C57BL/6J mouse, rep2
GSM2358182 Lung from untreated C57BL/6J mouse, rep3
BioProject PRJNA350206
SRA SRP091950

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89064_edgeR_miR.xls.gz 424.1 Kb (ftp)(http) XLS
GSE89064_miRNA_vs_blood_spearman_correlations.xlsx 216.7 Kb (ftp)(http) XLSX
GSE89064_reads_miR.txt.gz 98.3 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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