GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE89260 Query DataSets for GSE89260
Status Public on Nov 30, 2016
Title A somatic piRNA pathway in the Drosophila fat body ensures metabolic homeostasis and normal lifespan
Organisms Drosophila melanogaster; Drosophila simulans; Drosophila yakuba
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary In gonadal tissues, the Piwi-interacting (piRNA) pathway preserves genomic integrity by employing 23-29nt small RNAs complexed with argonaute proteins to suppress parasitic mobile sequences of DNA called transposable elements (TEs). While recent evidence suggests that the piRNA pathway may be present in select somatic cells outside the gonads, the role of a non-gonadal somatic piRNA pathway is not well characterized. Here we report a functional somatic piRNA pathway in the adult Drosophila fat body including the presence of the piRNA effector protein Piwi and canonical 23-29nt long TE-mapping piRNAs. piwi mutants exhibit depletion of fat body piRNAs, increased TE mobilization, increased levels of DNA damage, and reduced lipid stores. These mutants are starvation-sensitive, immunologically compromised, and short-lived, all phenotypes associated with compromised fat body function. These findings demonstrate the presence of a functional non-gonadal somatic piRNA pathway in the adult fat body that impacts normal metabolism and overall organismal health.
Overall design RNA-seq: 18 samples total, 6 tissues/genotypes (wild-type head, thorax, abdomen, ovary; piwi2/CyO fat body; and piwi2/piwi2 fat body), 3 replicates each.
Small RNA-seq: 6 samples total (wild-type, D. simulans, D. yakuba, ovoD1 mutant, piwi2/CyO, and piwi2/piwi2; all fat body).
Contributor(s) Jones BC, Wood JG, Helfand SL
Citation(s) 28000665
Submission date Oct 27, 2016
Last update date May 15, 2019
Contact name Jason G Wood
Organization name Brown University
Department MCB
Lab Helfand
Street address 185 Meeting St.
City Providence
State/province RI
ZIP/Postal code 02912
Country USA
Platforms (3)
GPL17275 Illumina HiSeq 2500 (Drosophila melanogaster)
GPL22293 Illumina HiSeq 2500 (Drosophila simulans)
GPL22610 Illumina HiSeq 2500 (Drosophila yakuba)
Samples (24)
GSM2363284 Head, rep1
GSM2363285 Head, rep2
GSM2363286 Head, rep3
BioProject PRJNA350849
SRA SRP092210

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89260_head.thorax.abdomen.ovary.easyRNAseq.rpkm.txt.gz 1.3 Mb (ftp)(http) TXT
GSE89260_head.thorax.abdomen.ovary.rawcounts.txt.gz 359.4 Kb (ftp)(http) TXT
GSE89260_piwi.pirna.repenrich.antisense.normalized.log2FC.txt.gz 5.3 Kb (ftp)(http) TXT
GSE89260_piwi.pirna.repenrich.antisense.rawcounts.txt.gz 2.3 Kb (ftp)(http) TXT
GSE89260_piwi.rnaseq.repenrich.counts.txt.gz 4.5 Kb (ftp)(http) TXT
GSE89260_piwi.rnaseq.repenrich.edgeR.txt.gz 25.6 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap