 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 30, 2016 |
| Title |
A somatic piRNA pathway in the Drosophila fat body ensures metabolic homeostasis and normal lifespan |
| Organisms |
Drosophila melanogaster; Drosophila simulans; Drosophila yakuba |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
In gonadal tissues, the Piwi-interacting (piRNA) pathway preserves genomic integrity by employing 23-29nt small RNAs complexed with argonaute proteins to suppress parasitic mobile sequences of DNA called transposable elements (TEs). While recent evidence suggests that the piRNA pathway may be present in select somatic cells outside the gonads, the role of a non-gonadal somatic piRNA pathway is not well characterized. Here we report a functional somatic piRNA pathway in the adult Drosophila fat body including the presence of the piRNA effector protein Piwi and canonical 23-29nt long TE-mapping piRNAs. piwi mutants exhibit depletion of fat body piRNAs, increased TE mobilization, increased levels of DNA damage, and reduced lipid stores. These mutants are starvation-sensitive, immunologically compromised, and short-lived, all phenotypes associated with compromised fat body function. These findings demonstrate the presence of a functional non-gonadal somatic piRNA pathway in the adult fat body that impacts normal metabolism and overall organismal health.
|
| |
|
| Overall design |
RNA-seq: 18 samples total, 6 tissues/genotypes (wild-type head, thorax, abdomen, ovary; piwi2/CyO fat body; and piwi2/piwi2 fat body), 3 replicates each.
Small RNA-seq: 6 samples total (wild-type, D. simulans, D. yakuba, ovoD1 mutant, piwi2/CyO, and piwi2/piwi2; all fat body).
|
| |
|
| Contributor(s) |
Jones BC, Wood JG, Helfand SL |
| Citation(s) |
28000665 |
| |
| Submission date |
Oct 27, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jason G Wood |
| E-mail(s) |
Jason_Wood@Brown.edu
|
| Organization name |
Brown University
|
| Department |
MCB
|
| Lab |
Helfand
|
| Street address |
185 Meeting St.
|
| City |
Providence |
| State/province |
RI |
| ZIP/Postal code |
02912 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
| GPL22293 |
Illumina HiSeq 2500 (Drosophila simulans) |
| GPL22610 |
Illumina HiSeq 2500 (Drosophila yakuba) |
|
| Samples (24)
|
| GSM2363287 |
Thorax, rep1 |
| GSM2363288 |
Thorax, rep2 |
| GSM2363289 |
Thorax, rep3 |
| GSM2363290 |
Abdomen, rep1 |
| GSM2363291 |
Abdomen, rep2 |
| GSM2363292 |
Abdomen, rep3 |
| GSM2363293 |
ovary, rep1 |
| GSM2363294 |
ovary, rep2 |
| GSM2363295 |
ovary, rep3 |
| GSM2363296 |
piwi heterozygote, rep1 |
| GSM2363297 |
piwi heterozygote, rep2 |
| GSM2363298 |
piwi heterozygote, rep3 |
| GSM2363299 |
piwi homozygote, rep1 |
| GSM2363300 |
piwi homozygote, rep2 |
| GSM2363301 |
piwi homozygote, rep3 |
| GSM2363302 |
WT smRNA |
| GSM2363303 |
D.sim smRNA |
| GSM2363304 |
D.yak smRNA |
| GSM2363305 |
ovoD1 smRNA |
| GSM2363306 |
piwi heterozygote smRNA |
| GSM2363307 |
piwi homozygote smRNA |
|
| Relations |
| BioProject |
PRJNA350849 |
| SRA |
SRP092210 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE89260_head.thorax.abdomen.ovary.easyRNAseq.rpkm.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
| GSE89260_head.thorax.abdomen.ovary.rawcounts.txt.gz |
359.4 Kb |
(ftp)(http) |
TXT |
| GSE89260_piwi.pirna.repenrich.antisense.normalized.log2FC.txt.gz |
5.3 Kb |
(ftp)(http) |
TXT |
| GSE89260_piwi.pirna.repenrich.antisense.rawcounts.txt.gz |
2.3 Kb |
(ftp)(http) |
TXT |
| GSE89260_piwi.rnaseq.repenrich.counts.txt.gz |
4.5 Kb |
(ftp)(http) |
TXT |
| GSE89260_piwi.rnaseq.repenrich.edgeR.txt.gz |
25.6 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...