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Series GSE89265 Query DataSets for GSE89265
Status Public on Jun 05, 2017
Title H3K36 Methylation Regulates Nutrient Stress Response in S. cerevisiae by Enforcing Transcriptional Fidelity
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by high throughput sequencing
Summary Set2-mediated methylation of H3K36 (H3K36me) regulates a diverse number of activities including DNA repair, mRNA splicing and the suppression of inappropriate or ‘cryptic’ transcription. Here, we describe an unexpected connection between Set2-mediated H3K36me and the regulation of nutrient stress response. We find cells deleted for SET2 (set2∆) are sensitive to inhibitors of Tor1, Tor2 and MAP kinase pathways that regulate the nutrient response pathway. Further genetic and biochemical analyses confirm a role for Set2-mediated H3K36me in nutrient stress response. At the molecular level, set2∆ cells demonstrate a dysregulated genome-wide transcriptional response to nutrient stress. Remarkably, newly initiated and bi-directional transcription events within the bodies of genes develop in set2∆ cells during nutrient stress. Importantly, these antisense transcripts extend into the promoters of the genes they arise from, resulting in pervasive transcriptional interference. Our results suggest that Set2-enforced transcriptional fidelity is critical to the proper regulation highly-tuned transcription programs.
 
Overall design Examination of ribosome-depleted stranded RNA in WT or set2∆ in S. cerevisiae at 0, 30, 60, and 120 minutes after nutrient stress.
 
Contributor(s) McDaniel SL, Hepperla AJ, Huang J, Dronamraju R, Adams AT, Kulkarni VG, Davis IJ, Strahl BD
Citation(s) 28614721
Submission date Oct 28, 2016
Last update date May 15, 2019
Contact name Jeremy M Simon
E-mail(s) jeremy_simon@med.unc.edu
Phone (919) 966-4050
Organization name University of North Carolina at Chapel Hill
Department Neuroscience
Street address 7018A Mary Ellen Jones Building
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
 
Platforms (1)
GPL17342 Illumina HiSeq 2500 (Saccharomyces cerevisiae)
Samples (24)
GSM2363346 WT_0min_Rep1
GSM2363347 WT_0min_Rep2
GSM2363348 WT_0min_Rep3
Relations
BioProject PRJNA350898
SRA SRP092234

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89265_DESeq2_Results_Files.tar.gz 5.3 Mb (ftp)(http) TAR
GSE89265_RAW.tar 113.0 Mb (http)(custom) TAR (of WIG)
GSE89265_RPKM_Matrix.txt.gz 890.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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