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Series GSE89471 Query DataSets for GSE89471
Status Public on Dec 31, 2017
Title B cell activation and plasma cell differentiation are inhibited by de novo DNA methylation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary B cells provide humoral immunity by differentiating into antibody-secreting plasma cells, a process that requires cellular division and is linked to DNA hypomethylation. Conversely, little is known about how de novo deposition of DNA methylation affects B cell fate and function. Here we show that genetic deletion of the de novo DNA methyltransferases Dnmt3a and Dnmt3b (Dnmt3-deficient) in mouse B cells results in normal B cell development and maturation, but increased cell activation and expansion of the germinal center B cell and plasma cell populations upon immunization. Gene expression is mostly unaltered in naive and germinal center B cells, but dysregulated in Dnmt3-deficient plasma cells. Differences in gene expression are proximal to Dnmt3-dependent DNA methylation and chromatin changes, both of which coincide with E2A and PU.1-IRF composite-binding motifs. Thus, de novo DNA methylation limits B cell activation, represses the plasma cell chromatin state, and regulates plasma cell differentiation.
 
Overall design This SuperSeries is composed of the SubSeries listed below.

Refer to individual Series
Web link https://www.ncbi.nlm.nih.gov/pubmed/29765016/
 
Citation(s) 29765016
Submission date Nov 02, 2016
Last update date May 15, 2019
Contact name Benjamin G Barwick
E-mail(s) benjamin.barwick@emory.edu
Phone (404) 285-2964
Organization name Emory University
Department Hematology and Medical Oncology
Lab Barwick Lab
Street address 1365 Clifton Rd. NE WCI-C 4th Floor Benches 36-37
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (53)
GSM2371204 Dnmt3_702 [RNA-seq]
GSM2371205 Dnmt3_703 [RNA-seq]
GSM2371206 Dnmt3_704 [RNA-seq]
This SuperSeries is composed of the following SubSeries:
GSE89412 B cell differentiation is limited by de novo DNA methylation [RNA-seq]
GSE89467 B cell differentiation is limited by de novo DNA methylation [RRBS]
GSE89470 B cell differentiation is limited by de novo DNA methylation [ATAC-seq]
Relations
BioProject PRJNA352270

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