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Status |
Public on Dec 31, 2017 |
Title |
B cell activation and plasma cell differentiation are inhibited by de novo DNA methylation |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing Methylation profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing
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Summary |
B cells provide humoral immunity by differentiating into antibody-secreting plasma cells, a process that requires cellular division and is linked to DNA hypomethylation. Conversely, little is known about how de novo deposition of DNA methylation affects B cell fate and function. Here we show that genetic deletion of the de novo DNA methyltransferases Dnmt3a and Dnmt3b (Dnmt3-deficient) in mouse B cells results in normal B cell development and maturation, but increased cell activation and expansion of the germinal center B cell and plasma cell populations upon immunization. Gene expression is mostly unaltered in naive and germinal center B cells, but dysregulated in Dnmt3-deficient plasma cells. Differences in gene expression are proximal to Dnmt3-dependent DNA methylation and chromatin changes, both of which coincide with E2A and PU.1-IRF composite-binding motifs. Thus, de novo DNA methylation limits B cell activation, represses the plasma cell chromatin state, and regulates plasma cell differentiation.
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Overall design |
This SuperSeries is composed of the SubSeries listed below.
Refer to individual Series
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Web link |
https://www.ncbi.nlm.nih.gov/pubmed/29765016/
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Citation(s) |
29765016 |
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Submission date |
Nov 02, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Benjamin G Barwick |
E-mail(s) |
benjamin.barwick@emory.edu
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Phone |
(404) 285-2964
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Organization name |
Emory University
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Department |
Hematology and Medical Oncology
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Lab |
Barwick Lab
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Street address |
1365 Clifton Rd. NE WCI-C 4th Floor Benches 36-37
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City |
Atlanta |
State/province |
GA |
ZIP/Postal code |
30322 |
Country |
USA |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (53)
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This SuperSeries is composed of the following SubSeries: |
GSE89412 |
B cell differentiation is limited by de novo DNA methylation [RNA-seq] |
GSE89467 |
B cell differentiation is limited by de novo DNA methylation [RRBS] |
GSE89470 |
B cell differentiation is limited by de novo DNA methylation [ATAC-seq] |
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Relations |
BioProject |
PRJNA352270 |