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| Status |
Public on Sep 11, 2007 |
| Title |
Rat Brain-Heart-Liver Control Mix Self-Self Hybridization |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
Eight “self-self” hybridizations were prepared as follows. Total RNA was isolated from each of three rat livers and brains, and equal mass amounts of each of these six samples were combined to create a mixed RNA population. Cy3- and Cy5-labeled cDNA was generated from this RNA mixture using an aminoallyl nucleoside analog indirect labeling protocol. Specifically, eight separate 20 microgram aliquots of the total RNA mixture were reverse transcribed into cDNA in the presence of aminoallyl-dUTP and the resulting cDNA was then coupled to either Cy3 or Cy5 mono NHS ester (four reactions with each label).
Keywords: Self-Self Hybridization
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| |
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| Overall design |
Two Color Experiment Dye-Swap Design with identical biological sample and 4 technical replicates.
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| |
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| Contributor(s) |
Juskeviciute E |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 04, 2007 |
| Last update date |
Mar 17, 2012 |
| Contact name |
Egle Juskeviciute |
| Organization name |
Thomas Jefferson University
|
| Department |
Pathology
|
| Street address |
1020 Locust st. JAH 527
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA102381 |
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