GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE89515 Query DataSets for GSE89515
Status Public on Nov 04, 2016
Title A new model for long-range chromatin reorganization linked to enhancer activation [Agilent_RNA]
Organism Mus musculus
Experiment type Expression profiling by genome tiling array
Summary The most prominent model for long-range enhancer regulation involves direct enhancer-promoter interaction by looping out the intervening chromatin. Using a synthetic biology approach, we have determined that a chromatin unfolding bteween Shh and its enhancers is regulated specifically by the Shh-Brain-Enhancers and is mediated by the recruitment of Poly (ADP-Ribose) Polymerase 1. This ‘chromatin unfolding’ model represents a new mechanism of long-range enhancer-promoter communication in addition to the looping and tracking models.
RNA-on-chip for probing nuclear RNA on the Shh regulatory region on ESCs and NPC showed no spreading of nuclear RNA in the regulatory region upon Shh activation.
Overall design Comparison of ESCs and NPC nuclear RNA profiles on Shh region
Contributor(s) Benabdallah NS, Williamson I, Illingworth RS, Boyle S, Grimes GR, Therizols P, Bickmore WA
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Nov 03, 2016
Last update date Jun 13, 2018
Contact name Nezha Suzanne Benabdallah
Organization name German Cancer Research Center, DKFZ
Department Functional and Structural Genomics
Lab Soft-Tissue Sarcoma
Street address Im Neuenheimer Feld 581 (TP4)
City Heidelberg
ZIP/Postal code 69120
Country Germany
Platforms (1)
GPL22635 Agilent-076323 En2_2 Custom Tiling Array G4822A (Feature number version)
Samples (4)
GSM2375068 ESC R1
GSM2375069 ESC R2
GSM2375070 NPC R1
BioProject PRJNA352421

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89515_RAW.tar 12.1 Mb (http)(custom) TAR (of BEDGRAPH, TXT)
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap