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Series GSE89591 Query DataSets for GSE89591
Status Public on Jan 09, 2017
Title DNA sequence properties that determine susceptibility to epiallelic switching [Bisulfite-Seq]
Organism Arabidopsis thaliana
Experiment type Methylation profiling by high throughput sequencing
Summary Transgenerationally heritable epialleles are defined by the stable propagation of alternative transcriptional states through mitotic and meiotic cell cycles. Given that the propagation of DNA methylation at CpG sites, mediated in Arabidopsis by MET1, plays a central role in epigenetic inheritance, we examined genome-wide DNA methylation in partial and complete loss-of-function met1 mutants. We interpreted the data in relation to transgenerational epiallelic stability and provide evidence that DNA sequence features such as density of CpGs and genomic repetitiveness of the loci largely determine their susceptibility to epiallelic switching. The importance of this rule was confirmed by analyses of common epialleles in natural Arabidopsis accessions and verified in rice.
Overall design Examination of DNA methylation of 2 met1 mutant alleles, 2 replicates of F2 plants obtained by crossing met1-3 with WT, and 2 transgenic MET1 complemented met1-1 lines.
Contributor(s) Catoni M
Citation(s) 28069706
Submission date Nov 07, 2016
Last update date May 15, 2019
Contact name Marco Catoni
Organization name University of Birmingham
Department School of Biosciences
Street address Edgbaston
City Birmingham
ZIP/Postal code B15 2TT
Country United Kingdom
Platforms (3)
GPL11221 Illumina Genome Analyzer IIx (Arabidopsis thaliana)
GPL17639 Illumina HiSeq 2500 (Arabidopsis thaliana)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (10)
GSM2384978 WT
GSM2384979 met1-1
GSM2384980 met1-3
This SubSeries is part of SuperSeries:
GSE89592 DNA sequence properties that determine susceptibility to epiallelic switching
BioProject PRJNA352683
SRA SRP092754

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89591_RAW.tar 2.0 Gb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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