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Status |
Public on Jan 09, 2017 |
Title |
DNA sequence properties that determine susceptibility to epiallelic switching [Bisulfite-Seq] |
Organism |
Arabidopsis thaliana |
Experiment type |
Methylation profiling by high throughput sequencing
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Summary |
Transgenerationally heritable epialleles are defined by the stable propagation of alternative transcriptional states through mitotic and meiotic cell cycles. Given that the propagation of DNA methylation at CpG sites, mediated in Arabidopsis by MET1, plays a central role in epigenetic inheritance, we examined genome-wide DNA methylation in partial and complete loss-of-function met1 mutants. We interpreted the data in relation to transgenerational epiallelic stability and provide evidence that DNA sequence features such as density of CpGs and genomic repetitiveness of the loci largely determine their susceptibility to epiallelic switching. The importance of this rule was confirmed by analyses of common epialleles in natural Arabidopsis accessions and verified in rice.
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Overall design |
Examination of DNA methylation of 2 met1 mutant alleles, 2 replicates of F2 plants obtained by crossing met1-3 with WT, and 2 transgenic MET1 complemented met1-1 lines.
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Contributor(s) |
Catoni M |
Citation(s) |
28069706 |
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Submission date |
Nov 07, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Marco Catoni |
Organization name |
University of Birmingham
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Department |
School of Biosciences
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Street address |
Edgbaston
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City |
Birmingham |
ZIP/Postal code |
B15 2TT |
Country |
United Kingdom |
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Platforms (3) |
GPL11221 |
Illumina Genome Analyzer IIx (Arabidopsis thaliana) |
GPL17639 |
Illumina HiSeq 2500 (Arabidopsis thaliana) |
GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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Samples (10)
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This SubSeries is part of SuperSeries: |
GSE89592 |
DNA sequence properties that determine susceptibility to epiallelic switching |
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Relations |
BioProject |
PRJNA352683 |
SRA |
SRP092754 |