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Series GSE89609 Query DataSets for GSE89609
Status Public on Aug 30, 2017
Title Role of the MicroRNA Machinery in Fasting-Induced Gene Expression Changes and Longevity in C. elegans (miRNA)
Platform organism synthetic construct
Sample organism Caenorhabditis elegans
Experiment type Non-coding RNA profiling by array
Summary Intermittent fasting (IF), a dietary restriction regimen, extends the lifespans of C. elegans and mammals by inducing gene expression changes. How fasting induces gene expression changes and longevity remains unclear. MicroRNAs (miRNAs) are small non-coding RNAs (approximately 22 nucleotides) that repress gene expression, and the expression of several miRNAs has been reported to be altered by fasting. In this study, we examined the role of the miRNA machinery in fasting-induced transcriptional changes and longevity in C. elegans. Our miRNA array analyses revealed that the expression levels of numerous miRNAs changed in adult worms after 48 hours of fasting. In addition to these changes, miRNA-mediated silencing complex (miRISC) components, including Argonaute proteins and GW182 proteins, and the miRNA-processing enzyme Drosha/DRSH-1, were up-regulated by fasting. Our lifespan measurements demonstrated that IF-induced longevity was suppressed by knockout or knockdown of miRISC components and was completely inhibited by drsh-1 ablation. Remarkably, drsh-1 ablation inhibited the fasting-induced changes in the expression of the target genes of DAF-16, the insulin/IGF-1 signaling effector. Fasting-induced transcriptome alterations were substantially and modestly suppressed in the drsh-1 null mutant and the null mutant of ain-1, a gene encoding GW182, respectively. These results indicate that components of the miRNA machinery, especially the miRNA-processing enzyme Drosha, play an important role in mediating IF-induced longevity via the regulation of fasting-induced gene expression changes.
To examine the fasting-induced changes in miRNA expression in adult worms, we performed miRNA array experiments.
Overall design We synchronized WT (N2) worms and raised under normal conditions. The 2 day adult animals were transferred to the new plate with (Fed) or without food (Fasting). We collected 2 day adult animals and the 4 day animals under fed or fasting conditions, then we extracted total RNA from them and subject them to microarray.
Contributor(s) Kogure A, Uno M, Ikeda T, Nishida E
Citation(s) 28507100
Submission date Nov 07, 2016
Last update date Aug 30, 2017
Contact name Eisuke Nishida
Phone +81-75-753-4230
Organization name Graduate School of Biostudies, Kyoto University
Department Department of Cell and Developmental Biology
Street address Kitashirakawa, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8502
Country Japan
Platforms (1)
GPL21572 [miRNA-4] Affymetrix Multispecies miRNA-4 Array [ProbeSet ID version]
Samples (3)
GSM2385319 day2_adult
GSM2385320 day4_adult_fed
GSM2385321 day4_adult_fasting
This SubSeries is part of SuperSeries:
GSE89624 Role of the MicroRNA Machinery in Fasting-Induced Gene Expression Changes and Longevity in C. elegans
BioProject PRJNA352728

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89609_RAW.tar 2.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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