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Series GSE89829 Query DataSets for GSE89829
Status Public on Mar 01, 2018
Title Anopheles coluzzii larval response to two purified Pseudomonas aeruginosa phenazine pigments 1-HP and Pyo.
Platform organism Anopheles gambiae
Sample organism Anopheles coluzzii
Experiment type Expression profiling by array
Summary Pseudomonas aeruginosa, the type species of the Pseudomonas genus, is an environmental Gram negative bacterium, well-known for its ability to produce toxins, resist antibiotics, and opportunistically colonize various niches, including invertebrate and vertebrate hosts. P. aeruginosa produces redox active secondary metabolites called phenazines involved in quorum sensing, biofilm formation, virulence, and iron acquisition. Moreover, these colorful pigmented virulence factors act as ligands for the highly conserved aryl hydrocarbon receptor (AhR) thereby regulating antibacterial defenses in vertebrates. Pseudomonas spp. are some of the most frequently identified bacteria in larval and adult stages of wild mosquito populations. Here we investigated global transcriptional changes induced in A. coluzzii third instar larvae incubated with a sublethal concentration (50 µM) of 1-hydroxyphenazine (1-HP) or pyocyanin (Pyo) at 4 h and 8 h of continuous incubation by whole-genome DNA microarrays.
 
Overall design Two experimental replicates (containing 15 pooled third instar larvae each) of 1-hydroxyphenazine (1-HP, 50 µM), pyocyanin (Pyo, 50 µM) or DMSO (50 µM equivalent volume) control; two timepoints of 4 h and 8 h. Microarray experiments were performed as dual-color hybridizations with color-swap dye-reversal (=two technical replicates for each sample). 1-HP or Pyo treatment samples were hybridized with respective DMSO controls. Total samples: 3 treatments * 2 experimental replicates * 2 timepoints = 12 samples; Total hybridizations: 2 comparisons * 2 experimental replicates * 2 timepoints * 2 technical replicates = 16 hybridizations.
 
Contributor(s) Huegli PA, Levashina EA
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Submission date Nov 14, 2016
Last update date Mar 03, 2018
Contact name Philip Adrian Huegli
E-mail(s) huegli@mpiib-berlin.mpg.de
Organization name Max Planck Institute for Infection Biology
Department Vector Biology
Street address Charitéplatz 1
City Berlin
State/province Berlin
ZIP/Postal code 10117
Country Germany
 
Platforms (1)
GPL13155 Agilent-020449 Mosquito Gene Expression Microarray (Feature Number version)
Samples (16)
GSM2390117 Pyo vs. DMSO, 4 h Timepoint, Replicate 1
GSM2390118 DMSO vs. Pyo, 4 h Timepoint, Replicate 1
GSM2390119 Pyo vs. DMSO, 8 h Timepoint, Replicate 1
Relations
BioProject PRJNA353494

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89829_RAW.tar 234.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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