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Series GSE90061 Query DataSets for GSE90061
Status Public on Aug 22, 2017
Title Whole genome RNA-sequencing from root tips ofArabidopsis thalianalow-phosphate insensitive mutants under High and Low phosphate
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Phosphate limitation constrains plant development in natural and agricultural systems. Under phosphate-limiting conditions plants activate genetic, biochemical and morphological modifications to cope with phosphate starvation. One of the morphological modifications that plants induce under phosphate limitation is the arrest of primary root growth and it is induced by the root tip contact with low phosphate media. The sensitive to proton rhizotoxicity (stop1) and aluminium activate malate transporter 1 (almt1) mutants of Arabidopsis thaliana continue primary root growth under in vitro Pi-limiting conditions, thus, to get insight into the molecular components that control primary root growth inhibition under low phosphate conditions we extracted and sequenced mRNA from the root tips (2-3 mm from the root apex) of wild-type plants (Col-0 accession) and low-phosphate-insensitive mutants almt1 and stop1 grown under low and high phosphate conditions 5 days after germination using an RNA-seq methodology.
Overall design Analysis of 9 different treatments, 3 different genotypes (Col-0,stop1,almt1), 4 different Phosphate treatments (High Pi, Low Pi, Low Pi -Fe, Low Pi +M), 1 biological replicate for treatment
Contributor(s) Ojeda-Rivera JO, Herrera-Estrella LR, Mora-Macías J
Citation(s) 28400510
Submission date Nov 18, 2016
Last update date May 15, 2019
Contact name Luis Rafael Herrera-Estrella
Organization name Cinvestav - Langebio
Department Langebio
Lab Plant Physiology and Metabolic Engineering
Street address Km 9.6 Libramiento Norte Carretera León
City Irapuato
State/province Guanajuato
ZIP/Postal code 36821
Country Mexico
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (9)
GSM2396841 Col-0 +Pi
GSM2396842 Col-0 -Pi
GSM2396843 Col-0 -Pi -Fe
BioProject PRJNA354385
SRA SRP093662

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE90061_RAW.tar 1.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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