 |
 |
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Nov 22, 2016 |
Title |
Uterine glands impact uterine receptivity, luminal fluid homeostasis and blastocyst implantation |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
Uterine glands are essential for pregnancy in mice and likely humans, because they secrete or transport bioactive substances that regulate uterine receptivity for blastocyst implantation. In mice, the uterus becomes receptive to blastocyst implantation on day 4, but is refractory by day 5. Here, blastocysts could be recovered from progesterone-induced uterine gland (PUGKO) but not wildtype (WT) mice on day 5 post-mating. Anti-adhesive Muc1 protein and microvilli were present on the luminal epithelium of PUGKO but not WT uteri. A number of known uterine receptivity genes and gland-specific genes were altered in the PUGKO uterus. Next, the uterus and uterine luminal fluid (ULF) were obtained from WT and PUGKO mice on day 3, 4 and 5. Transcriptome analysis revealed that 580 genes were decreased in the PUGKO uterus, however ULF secrotome analysis revealed that many proteins and several amino acids were increased in the PUGKO ULF. Of note, many proteins encoded by many gland-specific genes were not identified in the ULF of WT mice. These results support the ideas that uterine glands secrete factors that regulate ULF homeostasis and interact with other cell types in the uterus to influence uterine receptivity and blastocyst implantation for the establishment of pregnancy.
|
|
|
Overall design |
Endometrium from wild-type and PUKGO mice were subjected to RNA sequencing from days 3, 4, and 5 of pseudopregnancy (n=4 per day per group)
|
|
|
Contributor(s) |
Kelleher AM, Burns GW, Behura S, Wu G, Spencer TE |
Citation(s) |
27905495 |
|
Submission date |
Nov 21, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Thomas Spencer |
E-mail(s) |
spencerte@missouri.edu
|
Organization name |
University of Missouri
|
Department |
Animal Sciences
|
Lab |
ASRC 158
|
Street address |
920 East Campus Drive
|
City |
Columbia |
State/province |
MO |
ZIP/Postal code |
65211 |
Country |
USA |
|
|
Platforms (1) |
GPL18635 |
Ion Torrent Proton (Mus musculus) |
|
Samples (24)
|
|
Relations |
BioProject |
PRJNA354416 |
SRA |
SRP093614 |
Supplementary file |
Size |
Download |
File type/resource |
GSE90101_DOPP3.csv.gz |
1.1 Mb |
(ftp)(http) |
CSV |
GSE90101_DOPP4.csv.gz |
1.2 Mb |
(ftp)(http) |
CSV |
GSE90101_DOPP5.csv.gz |
1.2 Mb |
(ftp)(http) |
CSV |
GSE90101_readme.txt.gz |
209 b |
(ftp)(http) |
TXT |
SRA Run Selector |
Processed data are available on Series record |
Raw data are available in SRA |
|
|
|
|
 |