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Series GSE90101 Query DataSets for GSE90101
Status Public on Nov 22, 2016
Title Uterine glands impact uterine receptivity, luminal fluid homeostasis and blastocyst implantation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Uterine glands are essential for pregnancy in mice and likely humans, because they secrete or transport bioactive substances that regulate uterine receptivity for blastocyst implantation. In mice, the uterus becomes receptive to blastocyst implantation on day 4, but is refractory by day 5. Here, blastocysts could be recovered from progesterone-induced uterine gland (PUGKO) but not wildtype (WT) mice on day 5 post-mating. Anti-adhesive Muc1 protein and microvilli were present on the luminal epithelium of PUGKO but not WT uteri. A number of known uterine receptivity genes and gland-specific genes were altered in the PUGKO uterus. Next, the uterus and uterine luminal fluid (ULF) were obtained from WT and PUGKO mice on day 3, 4 and 5. Transcriptome analysis revealed that 580 genes were decreased in the PUGKO uterus, however ULF secrotome analysis revealed that many proteins and several amino acids were increased in the PUGKO ULF. Of note, many proteins encoded by many gland-specific genes were not identified in the ULF of WT mice. These results support the ideas that uterine glands secrete factors that regulate ULF homeostasis and interact with other cell types in the uterus to influence uterine receptivity and blastocyst implantation for the establishment of pregnancy.
 
Overall design Endometrium from wild-type and PUKGO mice were subjected to RNA sequencing from days 3, 4, and 5 of pseudopregnancy (n=4 per day per group)
 
Contributor(s) Kelleher AM, Burns GW, Behura S, Wu G, Spencer TE
Citation(s) 27905495
Submission date Nov 21, 2016
Last update date May 15, 2019
Contact name Thomas Spencer
E-mail(s) spencerte@missouri.edu
Organization name University of Missouri
Department Animal Sciences
Lab ASRC 158
Street address 920 East Campus Drive
City Columbia
State/province MO
ZIP/Postal code 65211
Country USA
 
Platforms (1)
GPL18635 Ion Torrent Proton (Mus musculus)
Samples (24)
GSM2398219 3-1
GSM2398220 3-2
GSM2398221 3-3
Relations
BioProject PRJNA354416
SRA SRP093614

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Supplementary file Size Download File type/resource
GSE90101_DOPP3.csv.gz 1.1 Mb (ftp)(http) CSV
GSE90101_DOPP4.csv.gz 1.2 Mb (ftp)(http) CSV
GSE90101_DOPP5.csv.gz 1.2 Mb (ftp)(http) CSV
GSE90101_readme.txt.gz 209 b (ftp)(http) TXT
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Processed data are available on Series record
Raw data are available in SRA

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