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| Status |
Public on Jun 12, 2017 |
| Title |
RNA association patterns of Salmonella cold shock proteins direct discovery of essential virulence function of CspC and CspE |
| Organism |
Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The functions of many bacterial RNA-binding proteins remain obscure due to a lack of knowledge of their cellular ligands. While well-studied cold-shock protein A (CspA) family members are induced and function at low temperature, other are highly expressed in infection-relevant conditions. Here, RNA ligand profiling of all CspA-family members linked CspC and CspE with Salmonella virulence pathways. Phenotypic assays in vitro demonstrated a crucial role for these proteins in membrane stress, motility, and biofilm formation. Moreover, deletion of cspC and cspE fully attenuates Salmonella in systemic mouse infection. Our results highlight RNA-binding proteins as regulators of pathogenicity and potential targets of antimicrobial therapy.
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| Overall design |
RIP-seq of bacterial cold-shock proteins; in vitro RNA-seq; RNA-seq in cell culture
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| |
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| Contributor(s) |
Barquist L, Michaux C |
| Citation(s) |
28611217 |
| |
| Submission date |
Dec 09, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Lars Barquist |
| E-mail(s) |
lars.barquist@helmholtz-hiri.de
|
| Organization name |
Helmholtz Institute for RNA-based Infection Research
|
| Street address |
Josef-Schneider-Straße 2 / Bau D15
|
| City |
Würzburg |
| State/province |
Bayern |
| ZIP/Postal code |
97080 |
| Country |
Germany |
| |
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| Platforms (3) |
| GPL19729 |
Illumina HiSeq 2500 (Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344) |
| GPL20056 |
Illumina NextSeq 500 (Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344) |
| GPL22775 |
Illumina MiSeq (Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344) |
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| Samples (26)
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| Relations |
| BioProject |
PRJNA356844 |
| SRA |
SRP094837 |
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