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Series GSE9121 Query DataSets for GSE9121
Status Public on Mar 01, 2008
Title Genome-wide effects of acute progressive feed restriction in liver and white adipose tissue
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Acute progressive feed restriction (APFR) represents a specific form of caloric restriction in which feed availability is increasingly curtailed over a period of a few days to a few weeks. It is often used for control animals in toxicological and pharmacological studies on compounds causing body weight loss to equalize weight changes between experimental and control groups and thereby, intuitively, to also set their metabolic states to the same phase. However, scientific justification for this procedure is lacking. In the present study, we analyzed by DNA microarrays the impact on hepatic gene expression in rats of two APFR regimens that caused identical diminution of body weight (19%) but differed slightly in duration (4 vs. 10 days). In addition, white adipose tissue (WAT) was also subjected to the transcriptomic analysis on day-4. The data revealed that the two regimens led to distinct patterns of differentially expressed genes in liver, albeit some major pathways of energy metabolism were similarly affected (particularly fatty acid and amino acid catabolism). The reason for the divergence appeared to be entrainment by the longer APFR protocol of peripheral oscillator genes, which resulted in derailment of circadian rhythms and consequent interaction of altered diurnal fluctuations with metabolic adjustments in gene expression activities. WAT proved to be highly unresponsive to the 4-day APFR as only 17 mRNA levels were influenced by the treatment. This study demonstrates that body weight is a poor proxy of metabolic state and that the customary protocols of feed restriction can lead to rhythm entrainment.
Keywords: physiological state comparison
Overall design Thirteen feed restricted and thirteen control animals, each hybridized to a single array. Pre-processing was performed by group: all WAT samples together in one group, all four-day liver samples in a second group, and all ten-day liver samples in a third.
Contributor(s) Pohjanvirta R, Boutros PC, Moffat ID, Linden J, Wendelin D, Okey AB
Citation(s) 18394668
Submission date Sep 20, 2007
Last update date Jul 31, 2017
Contact name Paul C Boutros
Organization name Ontario Institute for Cancer Research
Street address 101 College Street, Suite 800
City Toronto
State/province Ontario
ZIP/Postal code M5G 0A3
Country Canada
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (26)
GSM230919 rat_adipose_day4_control_replicate1
GSM230920 rat_adipose_day4_control_replicate2
GSM230921 rat_adipose_day4_control_replicate3
BioProject PRJNA102657

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Supplementary file Size Download File type/resource
GSE9121_RAW.tar 1.3 Gb (http)(custom) TAR (of CEL, DAT, EXP)
Processed data included within Sample table

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