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Series GSE91387 Query DataSets for GSE91387
Status Public on Dec 10, 2016
Title Microglial-specific transcriptome changes following chronic alcohol consumption
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Microglia are fundamentally important immune cells within the central nervous system (CNS) that respond to environmental challenges to maintain normal physiological processes. Alterations in steady-state cellular function and over-activation of microglia can facilitate the initiation and progression of neuropathological conditions such as Alzheimer’s disease, Multiple Sclerosis, and Major Depressive Disorder. Alcohol consumption disrupts signaling pathways including both innate and adaptive immune responses that are necessary for CNS homeostasis. Unbiased RNA-Seq profiling was used to identify gene expression changes in isolated microglia in response to recurring bouts of voluntary alcohol drinking behavior. Gene coexpression analysis identified a coordinately regulated group of genes, unique to microglia, that collectively are associated with alcohol consumption. Several genes in this group were involved in toll-like receptor signaling and production of the inflammatory cytokine interferon-gamma. Coordinate expression of these genes is not ascertained from an admixture of CNS cell-types, underscoring the importance of examining isolated cellular populations to reveal systematic gene expression changes arising from mature microglia. We identified a distinctive microglial gene expression signature for neuroimmune responses related to alcohol consumption that provides valuable insight into microglia-specific changes underlying the development of substance abuse, as well as related CNS disorders.
 
Overall design We examined mRNA from both total homogenate (mixture of all cell types) and microglia from the cortex of control mice and mice that have undergone chronic voluntary ethanol consumption
 
Contributor(s) Robinson GR, Mayfield RD
Citation(s) 29101021
Submission date Dec 09, 2016
Last update date May 15, 2019
Contact name Gizelle Marin Robinson
E-mail(s) gizellemrobinson@gmail.com
Phone 781-985-3004
Organization name University of Texas at Austin
Department Waggoner Center for Alcohol and Addiction Research
Lab Harris Lab
Street address 2500 Speedway
City Austin
State/province TX
ZIP/Postal code 78712
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (48)
GSM2422351 E_TH_1
GSM2422352 E_TH_2
GSM2422353 E_TH_3
Relations
BioProject PRJNA356884
SRA SRP094853

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE91387_RAW.tar 4.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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