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| Status |
Public on Jul 31, 2017 |
| Title |
The impact of Tcf1/Lef1 deficiency and prostagladin E (PGE) treatment on leukemic stem cell (LSC) transcriptome |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Comparison of trasncriptome bewteen control LSCs and Tcf1/Lef1 deficient LSCs, LSCs treated with DMSO, PGE1 or PGE2.
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| Overall design |
Lineage negative BM cells were isolated from control or Tcf1/Lef1-deficient mice, and retrovirally transduced with p210-BCR-ABL retrovirus twice (at 24 hr interval), and 48 hrs after isolation, Lin-c-Kit+ Sca1+GFP+ cells were sorted as LSCs. The LSCs established from control BM cells were treated with DMSO, 20µM PGE1 or 20 µM PGE2 for 6 hrs.
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| Contributor(s) |
Xue H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 13, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Bing He |
| Organization name |
Children's Hospital of Philadelphia
|
| Lab |
Kai Tan
|
| Street address |
3501 Civic Center Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19074 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA357379 |
| SRA |
SRP095102 |
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