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Series GSE92369 Query DataSets for GSE92369
Status Public on Apr 29, 2018
Title Regulatory inversion in NAC networks steers the timing of age-dependent cell death in plants [Nanostring]
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Aging is a time-dependent biological phenomenon governed by complex networks of regulatory components and their transitions over lifetime. Yet, there have been limited efforts to pin down age-associated networks and map their dynamic characteristics onto aging phenotypes. Here, we built time-course genetic regulatory networks of NAM/ATAF/CUC (NAC) transcription factors during the course of leaf aging in Arabidopsis, using causal regulatory relationships among NACs identified from mutants of 49 aging-associated NACs. These temporal networks revealed a regulatory inversion from activating to repressive regulatory modes at a pre-senescent stage. The inversion was governed by three hub NACs, and their mutants conferred earlier aging with altered expression of reactive oxygen species and salicylic acid response genes. Overexpression of the hub NACs delayed the regulatory inversion, rendering delayed age-dependent cell death. We conclude that the regulatory inversion in NAC networks at a pre-senescent stage directs when age-dependent cell death should proceed in plants.
 
Overall design We measured mRNA expression of 49 NACs in wild type (col-0) and 49 knockout mutant lines of NACs (duplicate for each mutant) at 4 stages (14, 18, 22, and 26 days of leaf age)
434 samples consist of:
34 wild type samples (=10, 8, 8 and 8 replicates for the four time points, respectively)
16 samples of anac092 mutant, one of the ANAC gene (=4 replicates for each of four time points)
384 samples of 48 anac mutants including at3g12910 and ntl9 (=2 replicates for each of four time points).
 
Contributor(s) Kim H, Park J, Kim J, Kim J, Hong S, Kim J, Kim J, Woo H, Lim P, Hwang D, Nam H
Citation(s) 29735710
Submission date Dec 14, 2016
Last update date Jul 29, 2018
Contact name Ji-Hwan Park
E-mail(s) jhpark706@kribb.re.kr
Organization name Korea Research Institute of Bioscience and Biotechnology (KRIBB)
Lab Korean Bioinformation Center (KOBIC)
Street address 125 Gwahak-ro, Yuseong-gu
City Daejeon
ZIP/Postal code 34141
Country South Korea
 
Platforms (1)
GPL22796 Nanostring nCounter assay, mRNA signature for ANAC family of A.thaliana
Samples (434)
GSM2428273 D14_Col_A1
GSM2428274 D14_Col_A2
GSM2428275 D14_Col_A3
This SubSeries is part of SuperSeries:
GSE92371 Regulatory inversion in NAC networks steers the timing of age-dependent cell death in plants
Relations
BioProject PRJNA357454

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE92369_Matrix_raw_data1.txt.gz 13.0 Kb (ftp)(http) TXT
GSE92369_Matrix_raw_data2.txt.gz 12.0 Kb (ftp)(http) TXT
GSE92369_Matrix_raw_data3.txt.gz 12.5 Kb (ftp)(http) TXT
GSE92369_Matrix_raw_data4.txt.gz 13.1 Kb (ftp)(http) TXT
Processed data included within Sample table

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