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Series GSE93056 Query DataSets for GSE93056
Status Public on Jun 12, 2017
Title A Ras GTPase associated protein is involved in the phototropic and circadian photobiology responses in fungi
Organism Phycomyces blakesleeanus
Experiment type Expression profiling by high throughput sequencing
Summary Light is an environmental signal perceived by most eukaryotic organisms that can have major impacts on their growth and development. The MadC protein in the fungus Phycomyces blakesleeanus (Mucoromycotina) has been postulated to form part of the photosensory input for phototropism of the fruiting body sporangiophores, but the madC gene has remained unidentified since the 1960s when madC mutants were first isolated. In this study the madC gene was identified by positional cloning. All madC mutant strains contain loss-of-function point mutations within a gene predicted to encode a GTPase activating protein (GAP) for Ras. The madC gene complements the Saccharomyces cerevisiae Ras-GAP ira1 mutant and the encoded MadC protein interacts with P. blakesleeanus Ras homologs in yeast two-hybrid assays, indicating that MadC is a regulator of Ras signaling. Deletion of the homolog in the filamentous ascomycete Neurospora crassa affects the circadian clock output, yielding a pattern of asexual conidiation similar to a ras-1 mutant that is used in circadian studies in N. crassa. Thus, MadC is unlikely to be a photosensor, yet is a fundamental link in the photoresponses from blue light perceived by the conserved White Collar complex with Ras signaling in two distantly-related filamentous fungal species.
Overall design Differential expression in response to light from Phycomyces blakesleeanus, comparing the wild-type strain and a mutant strain that is unable to perform phototropism.
Contributor(s) Polaino S, Villalobos-Escobedo JM
Citation(s) 28322269
Submission date Jan 03, 2017
Last update date May 15, 2019
Contact name Alfredo Herrera-Estrella
Organization name LANGEBIO
Department LANGEBIO
Lab Lab 7
Street address Libramiento Norte Carretera Leon Km 9.6
City Irapuato
State/province Guanajuato
ZIP/Postal code 36821
Country Mexico
Platforms (1)
GPL22874 Illumina HiSeq 2500 (Phycomyces blakesleeanus)
Samples (12)
GSM2443080 MadC-mycelium-Dark-R1
GSM2443081 MadC-mycelium-Dark-R2
GSM2443082 MadC-mycelium-Dark-R3
BioProject PRJNA360058
SRA SRP095996

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Supplementary file Size Download File type/resource
GSE93056_MadC_L_vs_MadC_D.txt.gz 379.8 Kb (ftp)(http) TXT
GSE93056_WT_L_vs_WT_D.txt.gz 385.1 Kb (ftp)(http) TXT
GSE93056_matrix_counts.txt.gz 252.9 Kb (ftp)(http) TXT
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Processed data are available on Series record
Raw data are available in SRA

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