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Series GSE9344 Query DataSets for GSE9344
Status Public on Dec 23, 2007
Title Gene expression in bovine mammary gland in response to increased milking frequency as determined by microarray and SAGE
Organism Bos taurus
Experiment type Expression profiling by array
Expression profiling by SAGE
Summary Transcript profiling was performed by Affymetrix microarray analysis and SAGE to characterize changes in gene expression in the bovine mammary gland in response to 4× versus 2× daily milking during the first week of lactation. These changes in gene expression may contribute to the increased milk production observed in response to increased milking frequency. Our results indicate that increased milking frequency alters expression of genes in the mammary gland related to increased extracellular matrix remodeling, neovascularization, metabolism, cell proliferation and apoptosis.
Keywords: Physiological response to stimulus
 
Overall design Multiparous Holstein cows were milked either 2× daily (Control, n = 3) or 2× daily from day 1 to 3 of lactation, and 4× daily beginning at day 4 of lactation until day 21 post partum (IMF4, n = 4). The milking intervals were approximately 12 hours for Control cows and 9, 3, 9, and 3 hours, respectively, for IMF4 cows. Mammary biopsies were obtained at approximately day 7 of lactation for extraction of total RNA and subsequent transcript profiling my microarray (Affymetrix). Pooled Control and IMF4 RNA samples were used to synthesize two SAGE libraries using the Long SAGE kit by Invitrogen.
 
Contributor(s) Connor EE, Capuco AV, Siferd S, Van Tassell CP, Sonstegard TS, Fernandes V
Citation(s) 18671851
Submission date Oct 16, 2007
Last update date Dec 17, 2012
Contact name Erin E Connor
E-mail(s) erin.connor@ars.usda.gov
Phone 301-504-6104
Fax 301-504-8414
Organization name USDA-ARS, BARC
Department Animal and Natural Resources Institute
Lab Bovine Functional Genomics Laboratory
Street address 10300 Baltimore Avenue
City Beltsville
State/province MD
ZIP/Postal code 20705
Country USA
 
Platforms (2)
GPL223 SAGE:10:NlaIII:Bos taurus
GPL2112 [Bovine] Affymetrix Bovine Genome Array
Samples (9)
GSM237687 Mammary_biopsy_control_pooled (SAGE)
GSM237688 Mammary_biopsy_IMF4_pooled (SAGE)
GSM238331 cow_control_biological rep1
Relations
BioProject PRJNA103031

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9344_RAW.tar 13.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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