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Series GSE93749 Query DataSets for GSE93749
Status Public on Apr 19, 2017
Title Single-base resolution methylome of 5-Methylcytosine in human and mouse transcriptome
Organisms Homo sapiens; Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary mRNAs of siCTRL, siNSUN2 and ALYREF-RIP HeLa cells, and multiple mouse tissues were purified using oligo (dT)-conjugated magnetic beads followed by RiboMinus treatement. mRNAs were fragmented to ~200 nt and treated by Sodium bisulfite solution (pH 5.1) containing Hydroquinone. Bisulfite treated mRNAs were reverse transcribed to cDNA using ACT random hexamer primers. The cDNAs were subjected to libraries construction using KAPA Stranded mRNA-Seq Kit (KAPA) and performed sequencing on HiSeq2500 (Illumina) in pair-end mode, creating reads with a length of 125 bp. Sequencing chemistry v4 (Illumina) was used and every sample was sequenced at one lane.
Overall design Explore the single-base resolution methylome of 5-Methylcytosine in mammalian transcriptome
Contributor(s) Yang X, Yang Y, Sun B
Citation(s) 28418038
Submission date Jan 18, 2017
Last update date May 15, 2019
Contact name Bao-Fa Sun
Organization name Beijing Institute of Genomics (BIG) of Chinese Academy of Sciences (CAS)
Street address Da-Tun Road
City Beijing
ZIP/Postal code 100101
Country China
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (22)
GSM2461423 HeLa-siCTRL-BS-rep1
GSM2461424 HeLa-siCTRL-BS-rep2
GSM2461425 HeLa-siNSUN2-BS-rep1
This SubSeries is part of SuperSeries:
GSE93751 5-methylcytosine promotes mRNA export
BioProject PRJNA362514
SRA SRP097125

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Supplementary file Size Download File type/resource
GSE93749_Human_m5C_sites_information.xls.gz 1.8 Mb (ftp)(http) XLS
GSE93749_Mouse_m5C_sites_information.xls.gz 7.9 Mb (ftp)(http) XLS
GSE93749_m5C_sites_in_ALYREF-RIP.xls.gz 2.2 Mb (ftp)(http) XLS
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Raw data are available in SRA
Processed data are available on Series record

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