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Series GSE93761 Query DataSets for GSE93761
Status Public on Jan 20, 2017
Title Phosphate solubilization and gene expression of phosphate-solubilizing bacterium Burkholderia multivorans WS-FJ9 under different levels of soluble phosphate
Organism Burkholderia multivorans
Experiment type Expression profiling by high throughput sequencing
Summary Phosphate-solubilizing bacteria (PSB) have the ability to dissolve insoluble phosphate and enhance soil fertility. However, the growth and mineral phosphate solubilization of PSB could be affected by exogenous soluble phosphate and the mechanism has not been fully understood. In the present study, the growth and mineral phosphate-solubilizing characteristics of PSB strain Burkholderia multivorans WS-FJ9 were investigated at six levels of exogenous soluble phosphate (0, 0.5, 1, 5, 10 and 20 mM). The WS-FJ9 strain showed better growth at high levels of soluble phosphate. The phosphate-solubilizing activity of WS-FJ9 reduced as the soluble phosphate concentration increased, as well as the production of pyruvic acid. Transcriptome profiling of WS-FJ9 at three levels of exogenous soluble phosphate (0, 5 and 20 mM) identified 446 differentially expressed genes, among which 44 genes were continuously up-regulated when soluble phosphate concentration increased and 81 genes were continuously down-regulated. Some genes related to cell growth were continuously up-regulated which would account for the better growth of WS-FJ9 at high levels of soluble phosphate. Genes involved in glucose metabolism, including glycerate kinase, 2-oxoglutarate dehydrogenase, and sugar ABC-type transporter were continuously down-regulated which indicates that metabolic channeling of glucose towards phosphorylative pathway was negatively regulated by soluble phosphate.
 
Overall design In the present study, the growth and mineral phosphate-solubilizing characteristics of PSB strain Burkholderia multivorans WS-FJ9 were investigated at six levels of exogenous soluble phosphate (0, 0.5, 1, 5, 10 and 20 mM).Transcriptome profiling of WS-FJ9 at three levels of exogenous soluble phosphate (0, 5 and 20 mM) were analysised.
 
Contributor(s) Zeng Q, Wu X
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jan 18, 2017
Last update date May 15, 2019
Contact name Qingwei Zeng
E-mail(s) qwzeng2@gmail.com
Organization name Nanjing Forestry University
Street address 159 Longpan road
City Nanjing
ZIP/Postal code 210037
Country China
 
Platforms (1)
GPL22941 Illumina HiSeq 2000 (Burkholderia multivorans)
Samples (3)
GSM2461701 WS-FJ9-0
GSM2461702 WS-FJ9-5
GSM2461703 WS-FJ9-20
Relations
BioProject PRJNA362532
SRA SRP097080

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE93761_All-Unigene.fa.gz 3.1 Mb (ftp)(http) FA
GSE93761_RAW.tar 60.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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