GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE93845 Query DataSets for GSE93845
Status Public on Jul 18, 2017
Title Nuclear mTOR acts as a transcriptional integrator of the androgen signaling pathway in prostate cancer progression [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Whether the nuclear fraction of mTOR plays a role in prostate cancer (PCa) and can participate in direct transcriptional crosstalk with the androgen receptor (AR) is as yet unknown. The intersection of gene expression, DNA binding-events, and metabolic studies uncovered the existence of a nuclear mTOR-AR transcriptional axis dictating the metabolic rewiring and nutrient usage of PCa cells. In human clinical specimens, nuclear localization of mTOR was significantly associated with metastasis and castration-resistant PCa (CRPC), correlating with a sustained metabolic gene program governed by mTOR in that context. This study thus uncovers an unexpected function of mTOR and underscores a paradigm shift from AR to mTOR as being the master transcriptional regulator of cell metabolism during PCa progression.
We performed ChIP-seq of mTOR to study its role in LNCaP cells with or without androgen stimulation. The study was done twice independently; processed data are shown as a pool of either vehicle-treated samples or R1881-treated samples.
Overall design Cells were seeded in media deprived from steroids. 48h later, cell were treated with 10nM R1881 or vehicle (EtOH) for 48h before crosslink and ChIP.
Contributor(s) Audet-Walsh É, Giguère V
Citation(s) 28724614
Submission date Jan 19, 2017
Last update date May 15, 2019
Contact name Etienne Audet-Walsh
Organization name Université Laval
Department Molecular Medicine
Street address 2705 boulevard Laurier
City Québec City
State/province QC
ZIP/Postal code G1V 4G2
Country Canada
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (3)
GSM2463796 Control_mTOR_ChIP-seq.bam.bigwig
GSM2463797 R1881_mTOR_ChIP-seq.bam.bigwig
GSM2463798 Input_mTOR_ChIP-seq.bam.bigwig
This SubSeries is part of SuperSeries:
GSE93846 Nuclear mTOR acts as a transcriptional integrator of the androgen signaling pathway in prostate cancer progression
BioProject PRJNA362597
SRA SRP097165

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE93845_RAW.tar 130.0 Mb (http)(custom) TAR (of BEDGRAPH, CSV, XLSX)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap