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| Status |
Public on Jul 18, 2017 |
| Title |
Nuclear mTOR acts as a transcriptional integrator of the androgen signaling pathway in prostate cancer progression [ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Whether the nuclear fraction of mTOR plays a role in prostate cancer (PCa) and can participate in direct transcriptional crosstalk with the androgen receptor (AR) is as yet unknown. The intersection of gene expression, DNA binding-events, and metabolic studies uncovered the existence of a nuclear mTOR-AR transcriptional axis dictating the metabolic rewiring and nutrient usage of PCa cells. In human clinical specimens, nuclear localization of mTOR was significantly associated with metastasis and castration-resistant PCa (CRPC), correlating with a sustained metabolic gene program governed by mTOR in that context. This study thus uncovers an unexpected function of mTOR and underscores a paradigm shift from AR to mTOR as being the master transcriptional regulator of cell metabolism during PCa progression.
We performed ChIP-seq of mTOR to study its role in LNCaP cells with or without androgen stimulation. The study was done twice independently; processed data are shown as a pool of either vehicle-treated samples or R1881-treated samples.
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| Overall design |
Cells were seeded in media deprived from steroids. 48h later, cell were treated with 10nM R1881 or vehicle (EtOH) for 48h before crosslink and ChIP.
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| Contributor(s) |
Audet-Walsh É, Giguère V |
| Citation(s) |
28724614 |
| |
| Submission date |
Jan 19, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Etienne Audet-Walsh |
| E-mail(s) |
etienne.audet-walsh@crchudequebec.ulaval.ca
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| Organization name |
Université Laval
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| Department |
Molecular Medicine
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| Street address |
2705 boulevard Laurier
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| City |
Québec City |
| State/province |
QC |
| ZIP/Postal code |
G1V 4G2 |
| Country |
Canada |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE93846 |
Nuclear mTOR acts as a transcriptional integrator of the androgen signaling pathway in prostate cancer progression |
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| Relations |
| BioProject |
PRJNA362597 |
| SRA |
SRP097165 |
