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Series GSE93863 Query DataSets for GSE93863
Status Public on Dec 24, 2019
Title Differential Expression of the Transcription Factor GATA3 Specifies Lineage and Functions of Innate Lymphoid Cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Lymphoid tissue inducer (LTi) cells are regarded as a subset of innate lymphoid cells (ILCs). However, these cells are not derived from the ILC common progenitor, which generates other ILC subsets and is defined by the expression of the transcription factor PLZF. Here we examined transcription factor(s) determining the fate of LTi progenitor versus non-LTi ILC progenitor. Conditional deletion of Gata3 resulted in the loss of PLZF+ non-LTi progenitors but not the LTi progenitors that expressed the transcription factor RORγt. Consistently, PLZF+ non-LTi progenitors expressed high amounts of GATA3 whereas GATA3 expression was low in RORγt+ LTi progenitors. The generation of both progenitors required the transcriptional regulator Id2, which defines the common helper-like innate lymphoid progenitor, but not cytokine signaling. Nevertheless, low GATA3 expression was necessary for the generation of functionally mature LTi cells. Thus, differential expression of GATA3 determines the fates and functions of distinct ILC progenitors.
 
Overall design To identify transcriptome changes from common lymphoid progenitor (CLP) to common helper-like innate lymphoid progenitor (ChILP) with RNA-Seq; To identify unique genes expressed by PD-1+ non-LTi ILC progenitor and CCR6+ LTi progenitor with RNA-Seq.
 
Contributor(s) Zhong C, Zheng M, Cui K, Martins A, Hu G, Li D, Tessarollo L, Kozlov S, Keller J, Tsang J, Zhao K, Zhu J
Citation(s) 31882362
Submission date Jan 19, 2017
Last update date Mar 24, 2020
Contact name Gangqing Hu
E-mail(s) michael.hu@hsc.wvu.edu
Organization name West Virginia University
Department MicroBiology, Immunology, and Cell Biology
Lab 2072A, HSC North, Floor 2
Street address 64 Medical Center Drive
City Morgantown
State/province West Virginia
ZIP/Postal code 26506-9177
Country USA
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (8)
GSM2464255 RNA_ChILP_1
GSM2464256 RNA_CLP_1
GSM2464257 RNA_ChILP_2
Relations
BioProject PRJNA362614
SRA SRP097178

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Supplementary file Size Download File type/resource
GSE93863_RAW.tar 3.8 Mb (http)(custom) TAR (of RPKM)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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