|
| Status |
Public on Jan 23, 2017 |
| Title |
Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85 |
| Organism |
Fibrobacter succinogenes subsp. succinogenes S85 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Microorganisms are key components for plant biomass breakdown within rumen environments. Gram negative Fibrobacter succinogenes have been identified as being active and dominant cellulolytic members of the rumen. In this study, F. succinogenes type strain S85 was adapted for steady state growth in continuous culture at pH 5.75 and confirmed to grow in the range of pH 5.60-5.65, which is lower than has been reported previously. RNA-seq analysis revealed 268 and 829 genes were differentially expressed at pH 6.1 and 5.65 compared to pH 6.7, respectively. Resequencing analysis identified seven single nucleotide polymorphisms (SNPs) in the sufD, yidE, xylE, rlmM, mscL and dosC genes of acid tolerant strains. Due to the absence of a F. succinogenes genetic system, homologues in Escherichia coli were mutated and complemented and the resulting strains were assayed for acid survival. Complementation with wild-type or acid tolerant F. succinogenes sufD restored the function of E. coli SufDSE, suggesting a possible role F. succinogenes sufD was involved in acid homeostasis. Recent genetic engineering developments need to be adapted and applied in F. succinogenes to further our understanding of this species.
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| |
|
| Overall design |
Chia-wei,Wu
|
| |
|
| Contributor(s) |
Brown SD, Spike T, Klingeman DM |
| Citation(s) |
28536480 |
| |
| Submission date |
Jan 22, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Dawn Marie Klingeman |
| E-mail(s) |
klingemandm@ornl.gov
|
| Phone |
+18655763435
|
| Organization name |
Oak Ridge National Lab
|
| Department |
Biosciences Division
|
| Lab |
RNA Profiling
|
| Street address |
1 Bethel Valley Rd building 15056 Rm 366 MS 6038
|
| City |
Oak Ridge |
| State/province |
TN |
| ZIP/Postal code |
37831-6342 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL22970 |
Illumina MiSeq (Fibrobacter succinogenes subsp. succinogenes S85) |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA362814 |
| SRA |
SRP097575 |
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