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Series GSE94246 Query DataSets for GSE94246
Status Public on May 10, 2017
Title HAND2 Target Gene Regulatory Networks Control Cardiac Valve Development
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The HAND2 transcriptional regulator controls cardiac development and we uncover addition essential functions in the endothelial to mesenchymal transition (EMT) underlying cardiac cushion development in the atrioventricular canal (AVC). In Hand2-deficient mouse embryos, the EMT underlying AVC cardiac cushion formation is disrupted and we combined ChIP-Seq of embryonic hearts with transcriptome analysis of wild-type and mutants AVCs to identify the functionally relevant HAND2 target genes. The HAND2 target gene regulatory network (GRN) includes most genes with known functions in EMT processes and AVC cardiac cushion formation. One of these is Snai1, an EMT master regulator whose expression is lost from Hand2-deficient AVCs. Re-expression of Snai1 in mutant AVC explants partially restores this EMT and mesenchymal cell migration. Furthermore, the HAND2-interacting enhancers in the Snai1 genomic landscape are active in embryonic hearts and other Snai1-expressing tissues. These results show that HAND2 directly regulates the molecular cascades initiating AVC cardiac valve development.
Overall design Atrioventricular canals (AVCs) were dissected from wild-type and Hand2 d/d (null-mutant) mouse embryos between E9.0-E9.25 (18-23 somites) and total RNA was analyzed.
Contributor(s) Laurent F, Girdziusaite A, Gamart J, Barozzi I, Osterwalder M, Lopez-Rios J, Lincoln J, Akiyama J, Visel A, Zuniga A, Zeller R
Citation(s) 28538179
Submission date Jan 27, 2017
Last update date May 15, 2019
Contact name Iros Barozzi
Organization name Medical University Vienna
Street address Borschkegasse 8a
City Vienna
ZIP/Postal code 1090
Country Austria
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (7)
GSM2471491 e9.5 AVC Hand2 null-mutant (1)
GSM2471492 e9.5 AVC Hand2 null-mutant (2)
GSM2471493 e9.5 AVC Hand2 null-mutant (3)
BioProject PRJNA369123
SRA SRP098206

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE94246_RAW.tar 700.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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