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Series GSE94298 Query DataSets for GSE94298
Status Public on Jul 24, 2017
Title Stable Inheritance of Spontaneous Epialleles in Arabidopsis thaliana
Organism Arabidopsis thaliana
Experiment type Methylation profiling by high throughput sequencing
Summary This research uses consecutive generations of two independent mutation accumulation (MA) lines in model organism A. thaliana to understand transgenerational stability of epialleles via self-fertilization. With whole-genome bisulfite sequencing, regions of instability were identified and quantified. The vast majority of the methylated genome is stably inherited to offspring and the identified unstable regions do not change frequently between generations. Additionally, an epigenetic cross of two MA lines was created to understand inheritance patterns of epialleles via outcrossing in the absence of genetic variation. Whole-genome bisulfite sequencing was used to predict epigenotype of the offspring without single nucleotide polymorphisms. In regions of differential methylation between the parents, about half of regions show predictable inheritance.
Overall design Eight and ten nearly consequetive generations from Arabidopsis thaliana mutation accumulation lines; genetic cross of two individuals from different MA lines, parents, twenty F2s, individuals of two generations of single-seed decent from the parents
Contributor(s) Hofmeister BT, Schmitz RJ
Citation(s) 28814343
Submission date Jan 30, 2017
Last update date May 15, 2019
Contact name Robert J Schmitz
Organization name University of Georgia
Department Genetics
Street address B416 Davison Life Sciences
City Athens
State/province GA
ZIP/Postal code 30602
Country USA
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (36)
GSM2472646 Line 69-G18
GSM2472647 Line 69-G19
GSM2472648 Line 69-G21
BioProject PRJNA369265
SRA SRP098589

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Supplementary file Size Download File type/resource
GSE94298_RAW.tar 6.1 Gb (http)(custom) TAR (of TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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