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Series GSE94525 Query DataSets for GSE94525
Status Public on Dec 20, 2017
Title Microglia isolated from juvenile offspring of dams with allergic asthma exhibit methylation and transcriptional alterations to autism risk genes [RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Asthma is one of the most common chronic diseases among pregnant women, and symptoms often worsen during pregnancy. Dysregulation in immune responses during pregnancy, such as those that develop with chronic allergic asthma, increase the risk of a having a child with an Autism Spectrum Disorder (ASD). We recently developed a mouse model of maternal allergic asthma (MAA) that induces alterations in behavioral outcomes of the offspring including changes in sociability, repetitive and perseverative behaviors. Pregnancy is also a time when epigenetic changes help a static genome adapt to the maternal environment, and consequently activation of the maternal immune system may alter the regulation of gene expression in the developing fetal brain. Perinatal exposure to maternal asthma alters DNA methylation of immune-related genes in human infants, suggesting that maternal asthma has long-lasting effects on the offspring’s’ immune function. Here we used the genome-wide approaches of whole genome bisulfite sequencing to examine DNA methylation combined with RNA sequencing to examine gene expression in acutely isolated microglia from juvenile MAA offspring. Differential analysis revealed significant alterations in the epigenome of microglia from MAA compared to PBS treated control offspring and identified genes involved in controlling microglial sensitivity to the environment and shaping both synaptic and long-range neuronal connections in the developing brain. Genes with altered methylation and expression in MAA juvenile microglia significantly overlapped with those identified in adult cortex from offspring of a different maternal immune activation (polyIC) autism model and with a curated list of autism risk genes in human, supporting a role for microglia in the pathogenesis of ASD.
Overall design Whole transcriptome RNA sequencing were performed on acutely isolated microglia from female C57BL6/J mice whose mother's had either received maternal immune activation (gestational day9,12, and 17) or PBS treatment. 3 MAA and 4 PBS biological replicates
Contributor(s) Annie VC, Janine L
Citation(s) 29134693
Submission date Feb 06, 2017
Last update date Jul 25, 2021
Contact name Annie Vogel Ciernia
Phone 6048270752
Organization name University of British Columbia
Street address 2215 Wesbrook Mall room 4550, Centre for Brain Health
City Vancouver
State/province British Columbia
ZIP/Postal code V6T 2A1
Country Canada
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (8)
GSM2477582 MAA1 RNAseq
GSM2477583 MAA2 RNAseq
GSM2477584 MAA3 RNAseq
This SubSeries is part of SuperSeries:
GSE94569 Microglia isolated from juvenile offspring of dams with allergic asthma exhibit methylation and transcriptional alterations to autism risk genes
BioProject PRJNA371411
SRA SRP098884

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Supplementary file Size Download File type/resource
GSE94525_RAW.tar 1.5 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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