|
| Status |
Public on Mar 14, 2018 |
| Title |
RNA-Sequencing Analysis of the Effects of UVA Radiation on Keratinocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Normal human epidermal keratinocytes were treated with sham or UVA (20J/cm2) radiation in duplicate. 6h after irradiation, samples were purified using Qiagen Rneasy kit, RNA quality assessment was performed using Agilent Bioanalyzer, and library preparation was performed using oligodT selection. RNA-Seq was performed on Illumina HiSeq2000 platform with 50 bp single-end reads. Analysis used the Tuxedo pipeline. Results were validated by qRT-PCR.
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| |
|
| Overall design |
Transcriptome of sham and UVA-irradiated normal human epidermal keratinocytes
|
| |
|
| Contributor(s) |
Sample A, He Y |
| Citation(s) |
28724634 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 ES016936 |
Mechanisms of UVA-induced skin cancer |
UNIVERSITY OF CHICAGO |
Yu-Ying He |
| R01 ES024373 |
Autophagy and GG-NER in UVB-induced skin cancer |
UNIVERSITY OF CHICAGO |
Yu-Ying He |
|
| |
| Submission date |
Mar 14, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Yu-Ying He |
| E-mail(s) |
yyhe@medicine.bsd.uchicago.edu
|
| Organization name |
University of Chicago
|
| Department |
Medicine
|
| Street address |
5841 S Maryland Ave, AMB N011
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA379125 |
| SRA |
SRP101864 |
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