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Series GSE97602 Query DataSets for GSE97602
Status Public on Dec 31, 2017
Title Genome -wide profile of SMRTER binding in Drosophila wing imaginal discs
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary Several co-repressors interact directly with the DNA-binding protein CSL [Su(H) in Drosophila] and are proposed to keep target genes silenced in the absence of Notch activity. To investigate co-repressor activity in the context of this well defined signalling pathway, we analysed the genome-wide binding profile of the best-characterized CSL co-repressor in Drosophila, Hairless, in Kc cells and in wing imaginal discs. The binding profile in wing discs of a second CSL interacting repressor, SMRTER, was also analysed. There was significant overlap between Hairless and Su(H), both in Kc cells and in wing discs, where they were predominantly found in chromatin with active enhancer marks. The Hairless complex was widely present at some Notch regulated enhancers in the wing disc,but no binding was detected at others, indicating that it is not essential for silencing per se. Analysis of target enhancers confirmed differential requirements for Hairless. SMRTER binding significantly overlapped with Hairless, rather than complementing it, and many enhancers were apparently co-bound by both factors. Our analysis indicates that the actions of Hairless and SMRTER gate the enhancers to Notch activity and to Ecdysone signalling respectively, to ensure that the appropriate levels and timing of target gene expression are achieved.
Overall design Three replicates from Chromatin immunoprecipitation of SMRTER-YFP from wing imaginal discs, input and ChIP samples
Contributor(s) Bray S, Chan S, Fischer B, Russell S
Citation(s) 29155828
Submission date Apr 10, 2017
Last update date Oct 04, 2019
Contact name Sarah Bray
Organization name University of Cambridge
Department PDN
Street address Downing Street
City Cambridge
ZIP/Postal code CB2 3DY
Country United Kingdom
Platforms (1)
GPL6629 [DM_tiling2_MR] Affymetrix Drosophila Tiling 2.0R Array
Samples (4)
GSM2572875 SMR wing disc rep1
GSM2572876 SMR wing disc input rep1
GSM2572877 SMR wing disc rep2
This SubSeries is part of SuperSeries:
GSE97603 Genome-wide profile of Notch pathway co-repressors and their role in shaping the Notch response
BioProject PRJNA382409

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE97602_RAW.tar 179.2 Mb (http)(custom) TAR (of CEL, SGR)
GSE97602_WD_Smr_EGS_r1.sm_quant-all_Ringo_hw300v8.sgr.gz 20.6 Mb (ftp)(http) SGR
GSE97602_WD_Smr_EGS_r12.sm_quant-all_normalNullzero_hw300v8g200FDR1.bed.gz 8.5 Kb (ftp)(http) BED
Processed data provided as supplementary file
Processed data are available on Series record

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