|
Status |
Public on Oct 02, 2018 |
Title |
Spermatid-specific linker histone HILS1 is a poor condenser of DNA and chromatin, and preferentially associates with LINE L1 elements |
Organism |
Rattus norvegicus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
Summary |
Background: Eleven linker histone subtypes are reported in mammals. Linker histones establish and maintain higher order chromatin structure. HILS1 is a spermatid-specific linker histone and its expression overlaps with the chromatin reorganization process during spermiogenesis; however, role of HILS1 in chromatin restructuring is largely unknown. Method: Genome-wide occupancy of the rat HILS1 protein was studied by ChIP-sequencing of pulldown DNA isolated from nuclei of 50-60 days old rat testis, using anti HILS1 antibody. Conclusion: Present study revealed that HILS1 is preferentially associated Line L1 elements in rat spermatids.
|
|
|
Overall design |
Nuclei of elongating/condensing spermatids cell types of the Rat (Rattus norvegicus) testicular cells were used for this purpose. Two runs were made with two different samples having two replicates each with a control for the ChIP used in each run. Illumina HiSeq 1000 sequencing platform was used with 101nt read length and paired end read type.
|
|
|
Contributor(s) |
Mishra NL, Shalini V, Gupta N, Ghosh K, Suthar N, Bhaduri U, Rao MS |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
|
Submission date |
Apr 24, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Manchanahalli Rangaswamy Satyanarayana Rao |
E-mail(s) |
mrsrao@jncasr.ac.in
|
Phone |
9108871102
|
Organization name |
Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR)
|
Department |
Molecular Biology and Genetics Unit (MBGU)
|
Lab |
Chromatin Biology Lab (CBL)
|
Street address |
Jakkur, Bengaluru, Karnataka
|
City |
Bangalore |
State/province |
Karnataka |
ZIP/Postal code |
560064 |
Country |
India |
|
|
Platforms (1) |
GPL18469 |
Illumina HiSeq 1000 (Rattus norvegicus) |
|
Samples (3) |
|
Relations |
BioProject |
PRJNA384019 |
SRA |
SRP105072 |