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| Status |
Public on Oct 02, 2018 |
| Title |
Spermatid-specific linker histone HILS1 is a poor condenser of DNA and chromatin, and preferentially associates with LINE L1 elements |
| Organism |
Rattus norvegicus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Background: Eleven linker histone subtypes are reported in mammals. Linker histones establish and maintain higher order chromatin structure. HILS1 is a spermatid-specific linker histone and its expression overlaps with the chromatin reorganization process during spermiogenesis; however, role of HILS1 in chromatin restructuring is largely unknown.
Method: Genome-wide occupancy of the rat HILS1 protein was studied by ChIP-sequencing of pulldown DNA isolated from nuclei of 50-60 days old rat testis, using anti HILS1 antibody.
Conclusion: Present study revealed that HILS1 is preferentially associated Line L1 elements in rat spermatids.
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| Overall design |
Nuclei of elongating/condensing spermatids cell types of the Rat (Rattus norvegicus) testicular cells were used for this purpose. Two runs were made with two different samples having two replicates each with a control for the ChIP used in each run. Illumina HiSeq 1000 sequencing platform was used with 101nt read length and paired end read type.
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| Contributor(s) |
Mishra NL, Shalini V, Gupta N, Ghosh K, Suthar N, Bhaduri U, Rao MS |
| Citation missing |
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| Submission date |
Apr 24, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Manchanahalli Rangaswamy Satyanarayana Rao |
| E-mail(s) |
mrsrao@jncasr.ac.in
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| Phone |
9108871102
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| Organization name |
Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR)
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| Department |
Molecular Biology and Genetics Unit (MBGU)
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| Lab |
Chromatin Biology Lab (CBL)
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| Street address |
Jakkur, Bengaluru, Karnataka
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| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560064 |
| Country |
India |
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| Platforms (1) |
| GPL18469 |
Illumina HiSeq 1000 (Rattus norvegicus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA384019 |
| SRA |
SRP105072 |
