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Series GSE98121 Query DataSets for GSE98121
Status Public on Nov 13, 2017
Title The Epstein-Barr virus episome maneuvers between nuclear chromatin compartments during reactivation [ChIP-seq]
Organisms Homo sapiens; human gammaherpesvirus 4
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Using chromatin conformation capture methods, we learned that the latent episome of the human Epstein-Barr virus (EBV) displays preferential chromosome association that correlates with gene density. The episome avoids gene-rich chromosomes and favors gene-poor chromosomes. Kaposi’s sarcoma-associated herpesvirus behaves similarly, but human papillomavirus does not, suggesting limited evolutionary conservation of this strategy. Moreover, the strongest contacts we detected between the human genome and EBV episome localized to OriP, the latent origin of replication. This genetic element, and the EBNA1 protein that binds there, are sufficient to reconstitute chromosome association preferences of the entire episome. Upon reactivation from latency, however, these preferences are lost. Detailed mapping of changes in interchromosomal contacts reveal that the episome moves away from repressive heterochromatin and toward activating euchromatin. Our work adds three-dimensional relocalization to the molecular events that occur during the genetic switch from EBV latency to reactivation. The involvement of only a myriad of interchromosomal contacts also argues for a possible role of this type of long-range association in gene regulation.
Overall design ChIP-seq identification of binding sites for two EBV proteins on the episomal viral genome
Contributor(s) Moquin SA, Miranda JL
Citation(s) 29142137
Submission date Apr 24, 2017
Last update date Jul 25, 2021
Contact name JJ Miranda
Organization name Barnard College, Columbia University
Street address 3009 Broadway
City New York
ZIP/Postal code 10027
Country USA
Platforms (1)
GPL23185 Illumina HiSeq 2500 (Homo sapiens; Human gammaherpesvirus 4)
Samples (2)
GSM2587519 BZLF1 ChIP-seq
GSM2587520 EBNA1 ChIP-seq
This SubSeries is part of SuperSeries:
GSE98123 The Epstein-Barr virus episome maneuvers between nuclear chromatin compartments during reactivation
BioProject PRJNA384034
SRA SRP105084

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Supplementary file Size Download File type/resource
GSE98121_RAW.tar 1.9 Mb (http)(custom) TAR (of WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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