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| Status |
Public on Jan 15, 2019 |
| Title |
Flipping the switch on Sex-lethal expression: Sister of Sex-lethal antagonizes Sxl-dependent alternative splicing to maintain a male-specific gene expression pattern in Drosophila (RIP-Seq) |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
|
| Summary |
In Drosophila female development is governed by a single RNA-binding protein, Sex lethal (Sxl), that controls the expression of key factors involved in dosage compensation, germline homeostasis and the establishment of female morphology and behaviour. Functional Sxl protein is only synthesized in female flies. Its expression is established in the pre-cellular, female embryo by an X-chromosome counting mechanism and maintained by a positive feedback loop with Sxl controlling alternative splicing of its own transcript. In male flies this feedback loop is not activated, keeping Sxl expression in the ‘off’ state. But what protects males against accidental triggering of the self-sustaining feedback loop and Sxl protein production snowballing out of control? Here we identify Sister of sex lethal (Ssx) as a protein that antagonizes Sxl in autoregulatory splicing by competition for the same RNA elements. Male flies mutant for ssx exhibit a low level of productive Sxl mRNA splicing and in cultured Drosophila cells, Sxl-induced changes in alternative splicing can be reverted by the expression of Ssx. In sum, this demonstrates that Ssx helps to establish a stable, male-specific gene expression pattern by protecting male flies against triggering Sxl expression.
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| Overall design |
Identification of Drosophila Sister of Sex-lethal (Ssx) bound RNAs
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| Contributor(s) |
Moschall R, Rossbach O, Lehmann G, Kullmann L, Eichner N, Strauss D, Strieder N, Meister G, Krahn M, Medenbach J |
| Citation(s) |
30590805 |
| |
| Submission date |
Apr 25, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Gerhard Lehmann |
| E-mail(s) |
gerhard.lehmann@vkl.uni-regensburg.de
|
| Organization name |
University of Regensburg
|
| Department |
Biochemistry 1
|
| Street address |
Universitaetsstrasse 31
|
| City |
Regensburg |
| ZIP/Postal code |
93053 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE98189 |
Flipping the switch on Sex-lethal expression: Sister of Sex-lethal antagonizes Sxl-dependent alternative splicing to maintain a male-specific gene expression pattern in Drosophila |
|
| Relations |
| BioProject |
PRJNA384221 |
| SRA |
SRP105201 |
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